Protein Info for Pf1N1B4_2391 in Pseudomonas fluorescens FW300-N1B4
Annotation: Membrane-bound lytic murein transglycosylase A precursor (EC 3.2.1.-)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K08304, membrane-bound lytic murein transglycosylase A [EC: 3.2.1.-] (inferred from 92% identity to pfo:Pfl01_5273)Predicted SEED Role
"Membrane-bound lytic murein transglycosylase A precursor (EC 3.2.1.-)" (EC 3.2.1.-)
KEGG Metabolic Maps
- Ascorbate and aldarate metabolism
- Glycosaminoglycan degradation
- Nucleotide sugars metabolism
- Ubiquinone and menaquinone biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 3.2.1.-
Use Curated BLAST to search for 3.2.1.-
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A161Z7V9 at UniProt or InterPro
Protein Sequence (390 amino acids)
>Pf1N1B4_2391 Membrane-bound lytic murein transglycosylase A precursor (EC 3.2.1.-) (Pseudomonas fluorescens FW300-N1B4) MNSRFKAWRHNLAWTLPMVAVLAGCTGGDSSKPKTHALATYSSATWEALPAVSDNDLVAG FGSWRSACTRLKADPVWGGTCAASANVQPTAKDIRGFLKQNLDVYGLRAANDNPNGLITG YYEPVYPGSLTQTEEANIPVYGVPEDMIIVSLDSIYPELKGKRLRGRLEGRVLKPYDDAA TIETNGVKAPVIAWLTDPMNLQFLQIQGSGRIQLDDGRQLRIGYADQNGHPYRPIGRWLV DQGELKKEDVTMGAISTWAKANPARIPELLGSNPSYVFFTRNPDSNEGPRGSLNVPLTAG YSAAVDRKVIPLGSLLWLSTTRPDGSALVRPVAAQDTGGAIAGEVRADLFWGTGDAAGQL AGDMKQQGQIWMLWPKGAALPQVPQVADTL