Protein Info for Pf1N1B4_2373 in Pseudomonas fluorescens FW300-N1B4

Annotation: D-amino acid dehydrogenase small subunit (EC 1.4.99.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 413 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details PF01266: DAO" amino acids 4 to 395 (392 residues), 225.5 bits, see alignment E=4.4e-70 PF01494: FAD_binding_3" amino acids 4 to 62 (59 residues), 26.1 bits, see alignment E=1.5e-09 PF00890: FAD_binding_2" amino acids 5 to 48 (44 residues), 22.1 bits, see alignment 2.5e-08 PF13450: NAD_binding_8" amino acids 7 to 37 (31 residues), 28 bits, see alignment (E = 6.3e-10)

Best Hits

Swiss-Prot: 70% identical to DADA2_PSEAE: D-amino acid dehydrogenase 2 (dadA2) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K00285, D-amino-acid dehydrogenase [EC: 1.4.99.1] (inferred from 80% identity to pfo:Pfl01_5290)

Predicted SEED Role

"D-amino acid dehydrogenase small subunit (EC 1.4.99.1)" in subsystem Pyruvate Alanine Serine Interconversions or Respiratory dehydrogenases 1 (EC 1.4.99.1)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.4.99.1

Use Curated BLAST to search for 1.4.99.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166NPS1 at UniProt or InterPro

Protein Sequence (413 amino acids)

>Pf1N1B4_2373 D-amino acid dehydrogenase small subunit (EC 1.4.99.1) (Pseudomonas fluorescens FW300-N1B4)
MAQRVCIIGGGVIGLTTAYALVRDGIDVMLIEARDSFASETSFANGGQLSYRYVAPLADA
GVPLQALGWMLRGDSPLKLRPRLDPAQWRWMAAFLAACRRSVNQRNGAHLLRLALLSQAT
LQGWRDEDRLDGFDWRRNGKLVTFREAASFEHACHSLADPRQQQVLSRTECAQLEPALAE
APFVGAIYTPDEEVADCHAFCLQLVARLKASGRCEFLPGRTVTGIRHGDGAVQAVEMGAQ
VLPVEQLVIAAGHRSPALALPGMNLPLYPLKGYSLTMPIRTEHRAPELSITDYNRKIVYA
RIGAQLRVAAMVDIVGFDPALDPKRLALIKRQAQETLPNAGDYDAAIEWAGMRPATPSGV
PLIGATAYRNLWLNLGHGALGFTLACGSARLLSELIARRTPSIEMQGLAPRVA