Protein Info for Pf1N1B4_2332 in Pseudomonas fluorescens FW300-N1B4

Annotation: tRNA (guanine46-N7-)-methyltransferase (EC 2.1.1.33)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 241 TIGR00091: tRNA (guanine-N(7)-)-methyltransferase" amino acids 54 to 240 (187 residues), 199 bits, see alignment E=2.6e-63 PF02390: Methyltransf_4" amino acids 66 to 235 (170 residues), 186.8 bits, see alignment E=1.2e-59

Best Hits

Swiss-Prot: 98% identical to TRMB_PSEPF: tRNA (guanine-N(7)-)-methyltransferase (trmB) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K03439, tRNA (guanine-N7-)-methyltransferase [EC: 2.1.1.33] (inferred from 98% identity to pfo:Pfl01_5329)

MetaCyc: 61% identical to tRNA m7G46 methyltransferase (Escherichia coli K-12 substr. MG1655)
tRNA (guanine-N(7)-)-methyltransferase. [EC: 2.1.1.33]

Predicted SEED Role

"tRNA (guanine46-N7-)-methyltransferase (EC 2.1.1.33)" (EC 2.1.1.33)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.33

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A162AUN9 at UniProt or InterPro

Protein Sequence (241 amino acids)

>Pf1N1B4_2332 tRNA (guanine46-N7-)-methyltransferase (EC 2.1.1.33) (Pseudomonas fluorescens FW300-N1B4)
MTESNDTPIQTEEGEERQHRRIKSFVMRAGRMTEGQQRGLDQGTPLFVLPLADAPVDFDQ
VFGRSAPRSLEIGFGMGHSLLEMAAASPEQDFIGVEVHRPGVGALLNGVLTQGLTNLRVY
DCDAIEVLNRCIADNSLDRLMLFFPDPWHKSRHHKRRIVQASFAELVRSKLKVGGVLHMA
TDWEPYAEYMLEVMNVAPGYRNLAEDGKCVPRPTERPITKFERRGERLGHGVWDLKFEKQ
S