Protein Info for Pf1N1B4_2269 in Pseudomonas fluorescens FW300-N1B4

Annotation: Purine nucleoside phosphorylase (EC 2.4.2.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF04463: 2-thiour_desulf
  • amino acids 4 to 147 (144 residues), 145.4 bits, E=6.2e-47

Best Hits

Swiss-Prot: 46% identical to YBBK_BACSU: Uncharacterized protein YbbK (ybbK) from Bacillus subtilis (strain 168)

KEGG orthology group: None (inferred from 88% identity to pfo:Pfl01_5390)

Predicted SEED Role

"Purine nucleoside phosphorylase (EC 2.4.2.1)" in subsystem Adenosyl nucleosidases or Deoxyribose and Deoxynucleoside Catabolism or Purine conversions (EC 2.4.2.1)

MetaCyc Pathways

• purine nucleotides degradation II (aerobic) (11/11 steps found)
• superpathway of purine nucleotide salvage (13/14 steps found)
• adenosine nucleotides degradation II (5/5 steps found)
• adenine and adenosine salvage III (4/4 steps found)
• guanosine nucleotides degradation III (4/4 steps found)
• inosine 5'-phosphate degradation (4/4 steps found)
• purine deoxyribonucleosides degradation I (4/4 steps found)
• ureide biosynthesis (6/7 steps found)
• purine deoxyribonucleosides degradation II (3/3 steps found)
• purine ribonucleosides degradation (5/6 steps found)
• adenine and adenosine salvage I (2/2 steps found)
• guanine and guanosine salvage I (2/2 steps found)
• xanthine and xanthosine salvage (2/2 steps found)
• superpathway of purine deoxyribonucleosides degradation (5/7 steps found)
• adenine and adenosine salvage V (2/3 steps found)
• pyrimidine deoxyribonucleosides degradation (2/3 steps found)
• superpathway of guanine and guanosine salvage (2/3 steps found)
• superpathway of pyrimidine deoxyribonucleosides degradation (3/6 steps found)
• nucleoside and nucleotide degradation (halobacteria) (2/6 steps found)
• fluoroacetate and fluorothreonine biosynthesis (1/6 steps found)
• arsenic detoxification (mammals) (8/17 steps found)
• nucleoside and nucleotide degradation (archaea) (2/10 steps found)
• salinosporamide A biosynthesis (3/15 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Nicotinate and nicotinamide metabolism
• Purine metabolism
• Pyrimidine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.2.1

Sequence Analysis Tools

See A0A166NN02 at UniProt or InterPro

Protein Sequence (161 amino acids)

>Pf1N1B4_2269 Purine nucleoside phosphorylase (EC 2.4.2.1) (Pseudomonas fluorescens FW300-N1B4)
MQKILVSRCLLGHRVRYDGGASGPFNLLEQWIAEGRVVPLCPEVAGGLPTPRAAAEIPGG
QGGEVLDGLASVITTEGEDVSAEFLSGACQALELVQQHGIRIAVLKANSPSCGNLLTYDG
TFSGVKVNGEGVTAALLKRHGVQVFSELELAEAAQALATLD