Protein Info for Pf1N1B4_2195 in Pseudomonas fluorescens FW300-N1B4

Annotation: Small-conductance mechanosensitive channel

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 193 transmembrane" amino acids 15 to 36 (22 residues), see Phobius details amino acids 61 to 85 (25 residues), see Phobius details amino acids 91 to 116 (26 residues), see Phobius details PF00924: MS_channel_2nd" amino acids 105 to 178 (74 residues), 35.2 bits, see alignment E=5.5e-13

Best Hits

KEGG orthology group: None (inferred from 94% identity to pfo:Pfl01_5477)

Predicted SEED Role

"Small-conductance mechanosensitive channel"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166NLS6 at UniProt or InterPro

Protein Sequence (193 amino acids)

>Pf1N1B4_2195 Small-conductance mechanosensitive channel (Pseudomonas fluorescens FW300-N1B4)
MEAFKLPLPAGWGEPLWLVVQILLILLAGYVAQRVVAKCLTRLGERYPFPPQFFMPLRGG
LRWLIMGSALIFVLERLGVSATVLWTALSGFVAVAAVAFFAMWSVLSNLLCAILIFTVGP
FRIGDVVELVDTTDKPGVKGRVVAINLLYTTLIEAEELGTGSAMVQVPNSLFFQRSVRRW
RGTDVFPSNGFEK