Protein Info for Pf1N1B4_2153 in Pseudomonas fluorescens FW300-N1B4

Annotation: Multidrug and toxin extrusion (MATE) family efflux pump YdhE/NorM, homolog

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 465 transmembrane" amino acids 12 to 32 (21 residues), see Phobius details amino acids 52 to 75 (24 residues), see Phobius details amino acids 90 to 110 (21 residues), see Phobius details amino acids 128 to 147 (20 residues), see Phobius details amino acids 159 to 182 (24 residues), see Phobius details amino acids 190 to 210 (21 residues), see Phobius details amino acids 239 to 264 (26 residues), see Phobius details amino acids 273 to 296 (24 residues), see Phobius details amino acids 317 to 336 (20 residues), see Phobius details amino acids 355 to 372 (18 residues), see Phobius details amino acids 393 to 412 (20 residues), see Phobius details amino acids 422 to 441 (20 residues), see Phobius details PF01554: MatE" amino acids 18 to 176 (159 residues), 88 bits, see alignment E=2.9e-29 amino acids 244 to 410 (167 residues), 102.9 bits, see alignment E=7.7e-34 TIGR00797: MATE efflux family protein" amino acids 18 to 423 (406 residues), 240.7 bits, see alignment E=1.3e-75

Best Hits

Swiss-Prot: 73% identical to NORM_PSESM: Probable multidrug resistance protein NorM (norM) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: K03327, multidrug resistance protein, MATE family (inferred from 92% identity to pfo:Pfl01_5517)

Predicted SEED Role

"Multidrug and toxin extrusion (MATE) family efflux pump YdhE/NorM, homolog"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161Z9C1 at UniProt or InterPro

Protein Sequence (465 amino acids)

>Pf1N1B4_2153 Multidrug and toxin extrusion (MATE) family efflux pump YdhE/NorM, homolog (Pseudomonas fluorescens FW300-N1B4)
MQHPARIELWAILRLAGPLIASQLAHMLMVLTDTLMMARLSPEALAGGGLGAATYSFVSI
FCIGVIAAVGTLVAIRQGAGDIIGAARLTQAGLWLAWLMALVAGLLLWNLKPVLLLFGQT
ETNVQAAGQFLLILPFALPGYLSFMALRGFTSAIGRATPVMVISLAGTVANFLLNYALIT
GMFGLPKMGLVGIGLVTAIVANLMALALALHIRRHPAYDAYPLRQGLSRPNRQYLKELWR
LGLPIGGTYAVEVGLFAFAALCMGTMGSTQLAAHQIALQIVSVAFMIPAGLSYAITMRIG
QHYGAGQLLDARQAGRVGIAFGAAAMLGFAMVFWLLPHQLIGLFLDHNDPAFREVINLAV
SLLAVAAWFELFDGTQTIAMGCIRGLKDAKTTFLVGLGCYWLIGAPAAWWMAFHLNWGPT
GVWWGLALGLACAAVSLTLAFEWKMKRMIRREPSSGASFQIAQPD