Protein Info for Pf1N1B4_210 in Pseudomonas fluorescens FW300-N1B4

Annotation: Cytochrome c-type biogenesis protein DsbD, protein-disulfide reductase (EC 1.8.1.8)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 579 signal peptide" amino acids 1 to 19 (19 residues), see Phobius details transmembrane" amino acids 163 to 193 (31 residues), see Phobius details amino acids 205 to 228 (24 residues), see Phobius details amino acids 240 to 262 (23 residues), see Phobius details amino acids 284 to 313 (30 residues), see Phobius details amino acids 320 to 343 (24 residues), see Phobius details amino acids 355 to 375 (21 residues), see Phobius details amino acids 381 to 399 (19 residues), see Phobius details amino acids 411 to 432 (22 residues), see Phobius details PF11412: DsbD_N" amino acids 29 to 134 (106 residues), 85.7 bits, see alignment E=7.5e-28 PF13386: DsbD_2" amino acids 169 to 366 (198 residues), 30.5 bits, see alignment E=1e-10 PF02683: DsbD" amino acids 170 to 373 (204 residues), 42.6 bits, see alignment E=2e-14 PF13899: Thioredoxin_7" amino acids 470 to 539 (70 residues), 47.3 bits, see alignment E=5.6e-16 PF13098: Thioredoxin_2" amino acids 476 to 570 (95 residues), 28.9 bits, see alignment E=3.8e-10

Best Hits

Swiss-Prot: 70% identical to DSBD_PSEF5: Thiol:disulfide interchange protein DsbD (dsbD) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)

KEGG orthology group: K04084, thiol:disulfide interchange protein DsbD [EC: 1.8.1.8] (inferred from 84% identity to pfo:Pfl01_3936)

Predicted SEED Role

"Cytochrome c-type biogenesis protein DsbD, protein-disulfide reductase (EC 1.8.1.8)" in subsystem Biogenesis of c-type cytochromes or Periplasmic disulfide interchange (EC 1.8.1.8)

Isozymes

Compare fitness of predicted isozymes for: 1.8.1.8

Use Curated BLAST to search for 1.8.1.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161XEH2 at UniProt or InterPro

Protein Sequence (579 amino acids)

>Pf1N1B4_210 Cytochrome c-type biogenesis protein DsbD, protein-disulfide reductase (EC 1.8.1.8) (Pseudomonas fluorescens FW300-N1B4)
MRGFLVFLLWLFASLAQAQPGNNPFDRKPDFLPIEKAFIFTSERLASGETQLFWQIANGY
YLYQKRLKFDDLPAQYKPVLPEGEAHSDEFFGEQQVYRQGLELKIPAGASGQIKVGFQGC
ADAGLCYPPHTQVVYLGNTTMPSLLNEAPDQALASDLQQRALGWSLLVFFGLGLLLAFTP
CSLPMLPILAGLIVGSGATPKRGFALAGSYVISMALVYAAMGVLAALLGANLQALLQNPW
LLGSFAAVFVLLALPMFGFFELQLPVAVRDRLEHVSRNQRGGNLIGAGVLGALSGLLVGP
CMTAPLAGALLYIAQSGNALHGGLILFAMGIGIGIPLLLLVTVGNRFLPKPGAWMNLLKG
VFGFLFLATALLMLRPVLDESLWLGLCGALLLIAAYSAWKQSQGFGRIAHLFGASSLLLG
LWGSLLVVGAAGGSDDPYQPLQVYSAGRTDAAAPSIHDAFTTIKEPAVLQRELDAAKAQG
QWVLLDYYADWCVACKVMEKQVFGKPQVLQALSDVRLLRLDVTADNAASRELLGRYKVPG
PPSLLWIGTDGIERRSQRITGEVDADTFLQRWTTTRDAR