Protein Info for Pf1N1B4_21 in Pseudomonas fluorescens FW300-N1B4

Annotation: Probable hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 277 PF00561: Abhydrolase_1" amino acids 67 to 149 (83 residues), 38.6 bits, see alignment E=1.6e-13 PF12146: Hydrolase_4" amino acids 68 to 157 (90 residues), 39.4 bits, see alignment E=6.4e-14 PF12697: Abhydrolase_6" amino acids 69 to 188 (120 residues), 44.6 bits, see alignment E=4.2e-15

Best Hits

KEGG orthology group: None (inferred from 94% identity to pfo:Pfl01_1880)

Predicted SEED Role

"Probable hydrolase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161Z2W7 at UniProt or InterPro

Protein Sequence (277 amino acids)

>Pf1N1B4_21 Probable hydrolase (Pseudomonas fluorescens FW300-N1B4)
MNTLSWVRGVNGTLGWFAPKLVASKMRLAFMTPRELPPRDWELPLLAKSERITLRFGLSA
LRWGQGPAVLLMHGWEGRPTQFAALITALVDAGYTVVALDGPAHGRSPGREANVVLFARA
MLEAAAELPPLQAVIGHSMGGASAMLAVQLGLRTETLVSIAAPARILGVLRGFARYVRLP
PKARSAFIRQVEKDVGMRAAALDVAHYQLDMPGLIVHAEDDNFVPVKESQLIHEAWFDSR
LLRLEEGGHQRVLADPRVIDGVLSLLAGRSLQSRQSA