Protein Info for Pf1N1B4_2019 in Pseudomonas fluorescens FW300-N1B4

Annotation: Putrescine ABC transporter putrescine-binding protein PotF (TC 3.A.1.11.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 366 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF02030: Lipoprotein_8" amino acids 5 to 103 (99 residues), 20.3 bits, see alignment E=4.2e-08 PF01547: SBP_bac_1" amino acids 38 to 299 (262 residues), 59.3 bits, see alignment E=1.3e-19 PF13416: SBP_bac_8" amino acids 41 to 331 (291 residues), 108.4 bits, see alignment E=1.1e-34 PF13343: SBP_bac_6" amino acids 76 to 333 (258 residues), 66.4 bits, see alignment E=5.8e-22

Best Hits

Swiss-Prot: 59% identical to SPUD_PSEAB: Putrescine-binding periplasmic protein SpuD (spuD) from Pseudomonas aeruginosa (strain UCBPP-PA14)

KEGG orthology group: K11073, putrescine transport system substrate-binding protein (inferred from 94% identity to pba:PSEBR_a5580)

MetaCyc: 56% identical to putrescine ABC transporter periplasmic binding protein (Escherichia coli K-12 substr. MG1655)
ABC-25-RXN [EC: 7.6.2.11, 7.6.2.16]

Predicted SEED Role

"Putrescine ABC transporter putrescine-binding protein PotF (TC 3.A.1.11.2)" in subsystem Polyamine Metabolism (TC 3.A.1.11.2)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.6.2.11 or 7.6.2.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A162BLK1 at UniProt or InterPro

Protein Sequence (366 amino acids)

>Pf1N1B4_2019 Putrescine ABC transporter putrescine-binding protein PotF (TC 3.A.1.11.2) (Pseudomonas fluorescens FW300-N1B4)
MNRLKRLIVPVLYATVFSGAVHAEERTLRVYNWFDYITPKALEDFKAQNTQTKLVYDIFD
TNEALEAKLLTGNSGYDVVVPSNVFLAKQIEAGVFQPLDRSKLPNWNHLDPKLMKLIEAN
DPGNKFAVPYMYGTILIGFNPDKIKAALGDNAPVDSWDLIFKEENISKLKQCGVALLDSP
SEILPLALQHLGLDPNSKKPADYAKAEALLMKIRPYITYFHSSKYMADIANGDICVAVGY
SGSFSQAANRAKEAKNGVIVDMRLPKEGAPIWFDMLAIPKGAKNPEDAYTFINYLLQPQV
IAPVSDFVGYPNPNKDATEMVDPAIRNNPNLYPTEAAMSTLYTLQPLPRDAERARTRAWT
KIKSGT