Protein Info for Pf1N1B4_2002 in Pseudomonas fluorescens FW300-N1B4

Annotation: putative Glutathione-regulated potassium-efflux system protein KefB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 587 transmembrane" amino acids 6 to 24 (19 residues), see Phobius details amino acids 31 to 50 (20 residues), see Phobius details amino acids 58 to 76 (19 residues), see Phobius details amino acids 85 to 105 (21 residues), see Phobius details amino acids 119 to 138 (20 residues), see Phobius details amino acids 150 to 173 (24 residues), see Phobius details amino acids 190 to 212 (23 residues), see Phobius details amino acids 220 to 237 (18 residues), see Phobius details amino acids 243 to 261 (19 residues), see Phobius details amino acids 273 to 292 (20 residues), see Phobius details amino acids 298 to 320 (23 residues), see Phobius details amino acids 331 to 351 (21 residues), see Phobius details amino acids 357 to 377 (21 residues), see Phobius details amino acids 425 to 446 (22 residues), see Phobius details amino acids 466 to 484 (19 residues), see Phobius details amino acids 515 to 538 (24 residues), see Phobius details amino acids 544 to 563 (20 residues), see Phobius details PF00999: Na_H_Exchanger" amino acids 12 to 376 (365 residues), 221.1 bits, see alignment E=1.1e-69

Best Hits

KEGG orthology group: K03455, monovalent cation:H+ antiporter-2, CPA2 family (inferred from 95% identity to pfo:Pfl01_5648)

Predicted SEED Role

"putative Glutathione-regulated potassium-efflux system protein KefB" in subsystem Potassium homeostasis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161Z264 at UniProt or InterPro

Protein Sequence (587 amino acids)

>Pf1N1B4_2002 putative Glutathione-regulated potassium-efflux system protein KefB (Pseudomonas fluorescens FW300-N1B4)
MHAISFIQDLAVIMLIAGVVTVLFHRLKQPVVLGYIVAGFIIGPHTPPFSLIHDEATIKT
LAELGVIFLMFCLGLEFSLRKLFKVGATAFIAAFLEIVLMIWIGYEIGRWFDWNTMDSLF
LGAILAISSTTIIVKALNDLKMKNERFAQLIFGVLIVEDILGIGIIALLSSIAVSGTVSS
GEVFSTVGKLSLFMIVALVIGILLVPRLLAYVAKFDSNEMLLITVLGLCFGFCLLVVKLE
YSMVLGAFLIGAIMAESRQLLKIEQLIEPVRDLFSAIFFVAIGLMLDPMILLQYAWPIAV
ITVAVVLGKMLSCGLGAFIAGNDGRTSLRVGMGLSQIGEFSFIIAALGMTLQVTSNFLYP
VAVAVSVITTLLTPYLIRAADPLSIKLAAVMPQRLSRVLGMYGEWLRSIQPQGEGALLAS
MIRRILLQVGVNLALVIAIFLSGAFFAERMSVYLQAWVSDPSWQKALIWGGALLLSLPFL
IAAYRKLKALSMLLAEMGVKPEMAGRHTQRVRRVIAEVIPILSLLVIFLLLTALSASILP
TNKLLVLIVIVAAAVAALLWRWFIRVHTRMQVALLETLDNHKESSGH