Protein Info for Pf1N1B4_1982 in Pseudomonas fluorescens FW300-N1B4

Annotation: Cardiolipin synthetase (EC 2.7.8.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 479 transmembrane" amino acids 7 to 31 (25 residues), see Phobius details amino acids 38 to 58 (21 residues), see Phobius details TIGR04265: cardiolipin synthase" amino acids 22 to 479 (458 residues), 379.7 bits, see alignment E=1.3e-117 PF13396: PLDc_N" amino acids 22 to 60 (39 residues), 25.8 bits, see alignment 1.3e-09 PF13091: PLDc_2" amino acids 136 to 244 (109 residues), 30.5 bits, see alignment E=4.4e-11 amino acids 320 to 445 (126 residues), 105.7 bits, see alignment E=2.6e-34 PF00614: PLDc" amino acids 219 to 245 (27 residues), 26.1 bits, see alignment (E = 9.7e-10) amino acids 393 to 419 (27 residues), 31.2 bits, see alignment (E = 2.3e-11)

Best Hits

Swiss-Prot: 85% identical to CLSA_PSEF5: Cardiolipin synthase A (clsA) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)

KEGG orthology group: K06131, cardiolipin synthase [EC: 2.7.8.-] (inferred from 90% identity to pfo:Pfl01_5665)

Predicted SEED Role

"Cardiolipin synthetase (EC 2.7.8.-)" in subsystem Glycerolipid and Glycerophospholipid Metabolism in Bacteria (EC 2.7.8.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.8.-

Use Curated BLAST to search for 2.7.8.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166NHG9 at UniProt or InterPro

Protein Sequence (479 amino acids)

>Pf1N1B4_1982 Cardiolipin synthetase (EC 2.7.8.-) (Pseudomonas fluorescens FW300-N1B4)
MDYFGPHVFGYLIAILHTLGSIAAIHAVLTVRTAQGSIAWALSLLFIPYLTLIPYLVFGR
STFDGYIKARRQANEEMRKAISELNWRPWVEEALAARASSAYASLRAMPKLGRMPCLANN
EVHLLIDGQATFDAIFDAISNARQAVLIQFFIIHDDRLGQRLHTLLTKKAAEGVAIYLLY
DRIGSHSLPHSYVQPLRDAGIEVKAFATRSGWLNRFQVNFRNHRKIVVVDGIVGFVGGLN
VGDEYMGEKPPLAPWRDTHVQVRGPVVACMQESFAEDWFWAARSLPPLILPEVYPDHGVL
CQLLATGPADSYETCSLFFVEAIHAATERVWITTPYFIPDEAVFAALRLAVLRGVDVRIL
LPSRADHRIVYAASSLYAFEAVRAGVRLFRYQPGFMHQKVVLIDSEISAIGSANMDNRSF
RLNFEVMLLTVDSPFAAEVEQMLNDDFAQAHEIAKEESRETHRLQQIGMRIARLISPIL