Protein Info for Pf1N1B4_1980 in Pseudomonas fluorescens FW300-N1B4

Annotation: Probable cadmium-transporting ATPase (EC 3.6.3.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 636 transmembrane" amino acids 20 to 42 (23 residues), see Phobius details amino acids 52 to 74 (23 residues), see Phobius details amino acids 86 to 112 (27 residues), see Phobius details amino acids 251 to 269 (19 residues), see Phobius details amino acids 275 to 303 (29 residues), see Phobius details amino acids 574 to 595 (22 residues), see Phobius details amino acids 601 to 623 (23 residues), see Phobius details TIGR01512: cadmium-translocating P-type ATPase" amino acids 84 to 624 (541 residues), 484.4 bits, see alignment E=5.8e-149 TIGR01525: heavy metal translocating P-type ATPase" amino acids 84 to 622 (539 residues), 518.1 bits, see alignment E=5.4e-159 TIGR01494: HAD ATPase, P-type, family IC" amino acids 108 to 604 (497 residues), 194.3 bits, see alignment E=4.3e-61 PF00122: E1-E2_ATPase" amino acids 134 to 306 (173 residues), 176.5 bits, see alignment E=4e-56 PF00702: Hydrolase" amino acids 325 to 528 (204 residues), 56.6 bits, see alignment E=4.8e-19

Best Hits

KEGG orthology group: None (inferred from 91% identity to pfo:Pfl01_5667)

Predicted SEED Role

"Probable cadmium-transporting ATPase (EC 3.6.3.3)" in subsystem Cobalt-zinc-cadmium resistance (EC 3.6.3.3)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3

Use Curated BLAST to search for 3.6.3.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166NHE7 at UniProt or InterPro

Protein Sequence (636 amino acids)

>Pf1N1B4_1980 Probable cadmium-transporting ATPase (EC 3.6.3.3) (Pseudomonas fluorescens FW300-N1B4)
MTAQTAAAPSMLSSVEQRSAARQLTLAMLALGLLALGLIWRWLSPEQTGVSQLLLGFASL
LVAVPVMRSAWYSLRYPSLHGITDQLIALAMLGAWATGDLLTAALLPIIMIFGHVLEERS
VIGSQEAIHALGKLTRSHARKIQADGSVVEVDNGTLKAGDLVEVRAGDRVPADGRVLSGQ
ASLDTASITGESVPIEAGVGMPVFGGAINLDGLLRIEVTRTGNESTLGKVIALMQSAERS
KPPITRLLERYAGSYMVLVLLLAAVTWFVTNDAQAMLAVLVAACPCALVLSAPATAIAGV
AVAARHGILIRSSAFLEELADLTSLVVDKTGTLTFGTLRLQSIDSPLEDRSHVLKLAASL
GSASSHPVSRALAGLVTQEHFLLLSDIHERQGLGVVAMTEQGEAALGRPELFAQLGIATS
AVPEHDGPIAGLALNGEFLAWLLLADSIKPEARFALSELRELGLGRQLLLTGDRQSVAHT
LARNVGISDVEAQALPEDKLNRVLKEIGSGFRPMVVGDGINDSLALKAGVVGVAMGAGGA
DIALASADIVLIGSDLRRLGTCVRLSRQCRQTLQVNVIIGLGWTLAIVAFAAFGWLGVAG
AMIAALLHNLSTLLVLGNAGRLLRFQEPLLKLKDEV