Protein Info for Pf1N1B4_1945 in Pseudomonas fluorescens FW300-N1B4

Annotation: N-acetylglucosamine-1-phosphate uridyltransferase (EC 2.7.7.23) / Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01173: UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase
  • amino acids 4 to 451 (448 residues), 656.2 bits, E=1.3e-201
• PF01128: IspD
  • amino acids 5 to 126 (122 residues), 27.6 bits, E=7.2e-10
• PF00483: NTP_transferase
  • amino acids 6 to 196 (191 residues), 50.9 bits, E=4.9e-17
• PF12804: NTP_transf_3
  • amino acids 6 to 121 (116 residues), 79.5 bits, E=9.6e-26
• PF00132: Hexapep
  • amino acids 263 to 296 (34 residues), 31.5 bits, 3.1e-11
• PF14602: Hexapep_2
  • amino acids 281 to 313 (33 residues), 16.6 bits, see alignment (E = 1.6e-06)
  • amino acids 392 to 426 (35 residues), 19 bits, 2.8e-07

Best Hits

Swiss-Prot: 92% identical to GLMU_PSEPF: Bifunctional protein GlmU (glmU) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K04042, bifunctional UDP-N-acetylglucosamine pyrophosphorylase / Glucosamine-1-phosphate N-acetyltransferase [EC: 2.3.1.157 2.7.7.23] (inferred from 92% identity to pfo:Pfl01_5728)

MetaCyc: 56% identical to fused N-acetylglucosamine-1-phosphate uridyltransferase and glucosamine-1-phosphate acetyltransferase (Escherichia coli K-12 substr. MG1655)
UDP-N-acetylglucosamine diphosphorylase. [EC: 2.7.7.23]; Glucosamine-1-phosphate N-acetyltransferase. [EC: 2.7.7.23, 2.3.1.157]

Predicted SEED Role

"N-acetylglucosamine-1-phosphate uridyltransferase (EC 2.7.7.23) / Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157)" in subsystem Peptidoglycan Biosynthesis or Sialic Acid Metabolism or UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis (EC 2.3.1.157, EC 2.7.7.23)

MetaCyc Pathways

• O-antigen building blocks biosynthesis (E. coli) (10/11 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis I (5/5 steps found)
• peptidoglycan recycling I (11/14 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis II (4/6 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (4/7 steps found)
• chitin biosynthesis (5/9 steps found)
• superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (6/24 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (12/33 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Nucleotide sugars metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.157 or 2.7.7.23

Sequence Analysis Tools

See A0A161Z234 at UniProt or InterPro

Protein Sequence (455 amino acids)

>Pf1N1B4_1945 N-acetylglucosamine-1-phosphate uridyltransferase (EC 2.7.7.23) / Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157) (Pseudomonas fluorescens FW300-N1B4)
MSLEIVILAAGQGTRMRSALPKVLHPVAGNSMLGHVIHTARQLDPQRIHVVIGHGAEAVR
ERLAADDLNFVLQDQQLGTGHAVAQAVPFIESDTVLILYGDVPLIEVETLQRLLKLAAPQ
QLGLLTVELDDPTGYGRIVRNAAGKVTAIVEQKDANDAERAITEGNTGILAVPFERLGDW
MSRLSNNNAQGEYYLTDVIAMAVSDGLVVATEHPHDAMEVQGANDRKQLAELERHYQLRA
ARRLMAQGVTLRDPARFDVRGEVTVGRDVLIDINVILEGKVVIEDDVVIGPNCVIKDSTL
RKGVVIKANSHIEGAILGEGSDAGPFARLRPGTVLEAGAHVGNFVELKNAHMGEGAKAGH
LTYLGDAEIGARTNIGAGTITCNYDGANKWKTVLGEDVFIGSNNSLVAPVDISAGATTAA
GSTITQNVDKAQLAVGRARQKNIDGWKRPEKLKKS