Protein Info for Pf1N1B4_1910 in Pseudomonas fluorescens FW300-N1B4

Annotation: Rossmann fold nucleotide-binding protein Smf possibly involved in DNA uptake

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 366 transmembrane" amino acids 229 to 250 (22 residues), see Phobius details TIGR00732: DNA protecting protein DprA" amino acids 78 to 297 (220 residues), 254.2 bits, see alignment E=3.7e-80 PF02481: DNA_processg_A" amino acids 82 to 288 (207 residues), 249.2 bits, see alignment E=2.4e-78 PF17782: DprA_WH" amino acids 307 to 359 (53 residues), 30.8 bits, see alignment 2.4e-11

Best Hits

KEGG orthology group: K04096, DNA processing protein (inferred from 87% identity to pfo:Pfl01_0019)

Predicted SEED Role

"Rossmann fold nucleotide-binding protein Smf possibly involved in DNA uptake" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166NG65 at UniProt or InterPro

Protein Sequence (366 amino acids)

>Pf1N1B4_1910 Rossmann fold nucleotide-binding protein Smf possibly involved in DNA uptake (Pseudomonas fluorescens FW300-N1B4)
MSLSACTSISPAELEARLRLHRLPELGPARFKKLLEAFGSASKAISAPASAWRALGLPLA
CAEARRSSEIRDRASHALAWLERPGQHLLMWDQPDYPALLAQISDAPPLLFAAGDPGILE
KPQLAMVGSRRASRPGMDTAAAFSRTLAGAGFVITSGLALGIDAAAHQAALDVGGQTVGV
LGTGLENFYPQRNRRLADAMIASGSAVLSEFPLDAGPSPSNFPRRNRIISGLSLGVLVVE
ASVASGSLITARLAAEQGREVYAIPGSIHHPGAKGCHQLIRDGAVLVETIEHILEALRGW
QRLPLSTETPTETHPLLILLHAAPHTSEALAVTSGWALSKVLAALTELEMDGRAVCESGR
WFARVN