Protein Info for Pf1N1B4_1903 in Pseudomonas fluorescens FW300-N1B4

Annotation: Choline-sulfatase (EC 3.1.6.6)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 504 signal peptide" amino acids 7 to 16 (10 residues), see Phobius details TIGR03417: choline-sulfatase" amino acids 3 to 498 (496 residues), 857.8 bits, see alignment E=1.5e-262 PF00884: Sulfatase" amino acids 4 to 344 (341 residues), 170 bits, see alignment E=1.6e-53 PF16347: SGSH_C" amino acids 294 to 430 (137 residues), 50.3 bits, see alignment E=6.6e-17 PF12411: Choline_sulf_C" amino acids 445 to 497 (53 residues), 92.2 bits, see alignment 2.8e-30

Best Hits

KEGG orthology group: None (inferred from 93% identity to pba:PSEBR_a65)

Predicted SEED Role

"Choline-sulfatase (EC 3.1.6.6)" in subsystem Choline Transport or Choline and Betaine Uptake and Betaine Biosynthesis (EC 3.1.6.6)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.6.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161Z208 at UniProt or InterPro

Protein Sequence (504 amino acids)

>Pf1N1B4_1903 Choline-sulfatase (EC 3.1.6.6) (Pseudomonas fluorescens FW300-N1B4)
MKRKNILFIMADQMAAPMLPFYGPSPIKLPNLSRLAAEGVVFDAAYCNSPLCAPSRFTLV
SGQLPSKIGAYDNAADFPADVPTYAHYLRRLGYRTALSGKMHFCGPDQLHGYEERLTSDI
YPADYGWSVNWDEPDVRPTWYHNMSSVLQAGPCVRTNQLDFDEEVVFKAQQYLFDHIRED
GDQPFCLTVSMTHPHDPYTIPKTFWDMYDDADIPLPETPAQDQLDPHSQRLLKVYDLWDK
PLPVDKIRDARRAYFGACSYIDSNVGKLLQTLEETGLIDDTIIVFSGDHGDMLGERGLWY
KMHWYEMAARVPLLISAPGQFGAGRVSAAVSTADLLPTFVELAGGSLEPGLPLDGRSLVS
HLQGQGGHDEVFGEYMAEGTISPLMMIRRGAYKFIYSEDDPCLLFDVHNDPKEQEELSQS
PQYRQLFDEFLAQARAKWDIPSIHQQVLASQRRRRFVADALTIGKLKSWDHQPLVDASQQ
YMRNHIDLDDLERKARYPQPCQNQ