Protein Info for Pf1N1B4_1895 in Pseudomonas fluorescens FW300-N1B4

Annotation: Metalloprotease, insulinase family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 459 signal peptide" amino acids 1 to 19 (19 residues), see Phobius details transmembrane" amino acids 434 to 454 (21 residues), see Phobius details PF00675: Peptidase_M16" amino acids 66 to 149 (84 residues), 46 bits, see alignment E=5.6e-16 PF05193: Peptidase_M16_C" amino acids 190 to 362 (173 residues), 67.1 bits, see alignment E=1.9e-22

Best Hits

KEGG orthology group: None (inferred from 84% identity to pfo:Pfl01_0035)

Predicted SEED Role

"Metalloprotease, insulinase family"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166NFV4 at UniProt or InterPro

Protein Sequence (459 amino acids)

>Pf1N1B4_1895 Metalloprotease, insulinase family (Pseudomonas fluorescens FW300-N1B4)
MRCLLFACLLLGSLPSFALDRFQVEGYTLRNGLQLVLKPGTERGHVAIRLVVGVGLDDFS
CADKELPHLLEHLLFSGIDATGEGGLEERMQALGGEWNAYTSNADTTFVIEAPAKNQRKV
LDLLLALLTQTRIDDNAINVAKQVVEREDGGHYSHLQRWLDRQDLGHTASNQLAVELGLK
CPERAQVDHLTREQLEKVRKAWYAPNNMTLIVVGDLDRLLPAYLERAYGALEAVEPSTHL
PLPDIQASAAHERNLSNGFVGGGAKLHWLVPEPVLEDQHDETFDLLKDYLDWALYRQLRL
AHGLSYGPSAEREVFGGVGFMSLNADLDRDDVLAAEQVLDELKADLLKNGLDAATFARLK
QAAIARQAWAVQGNSALADYYWSALGDYEDGHFLNPAKALQDVTLAEANKAMRELLLQPG
YLRIEKPLMSYDQVMWAIVGGFGLMVLGILGWRVHRRKK