Protein Info for Pf1N1B4_1879 in Pseudomonas fluorescens FW300-N1B4

Annotation: Periplasmic thiol:disulfide interchange protein DsbA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 213 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details PF13462: Thioredoxin_4" amino acids 45 to 180 (136 residues), 39.7 bits, see alignment E=8.6e-14 PF01323: DSBA" amino acids 95 to 186 (92 residues), 61.2 bits, see alignment E=1.8e-20

Best Hits

Swiss-Prot: 81% identical to DSBA_PSESM: Thiol:disulfide interchange protein DsbA (dsbA) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: K03673, thiol:disulfide interchange protein DsbA (inferred from 93% identity to pba:PSEBR_a43)

Predicted SEED Role

"Periplasmic thiol:disulfide interchange protein DsbA" in subsystem Biogenesis of c-type cytochromes or Periplasmic disulfide interchange

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A162BL82 at UniProt or InterPro

Protein Sequence (213 amino acids)

>Pf1N1B4_1879 Periplasmic thiol:disulfide interchange protein DsbA (Pseudomonas fluorescens FW300-N1B4)
MRNLIISAALVAASLFGMTAQAAEKPEAPYVELSNPVPVAAPGKIEVVELFWYGCPHCYA
FEPVINPWVEKLPSDVNFVRIPAMFGGPWDAHGQMFLTLEAMGVESKVHAAVFEAIQKEH
KKLTDKEDMADFLATQGVDKDKFLATFDSFAIKGQINKAKELAKKYEITGVPTMIVNGKY
RFDIGSAGGAEQALKLADQLVAKERAATKAVAN