Protein Info for Pf1N1B4_1875 in Pseudomonas fluorescens FW300-N1B4

Annotation: DNA polymerase I (EC 2.7.7.7)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 922 TIGR00593: DNA polymerase I" amino acids 6 to 922 (917 residues), 937.8 bits, see alignment E=4.1e-286 PF02739: 5_3_exonuc_N" amino acids 6 to 167 (162 residues), 180 bits, see alignment E=6.1e-57 PF01367: 5_3_exonuc" amino acids 168 to 270 (103 residues), 102.4 bits, see alignment E=3.3e-33 PF01612: DNA_pol_A_exo1" amino acids 315 to 509 (195 residues), 95.4 bits, see alignment E=7e-31 PF00476: DNA_pol_A" amino acids 547 to 920 (374 residues), 544.3 bits, see alignment E=3e-167

Best Hits

Swiss-Prot: 79% identical to DPO1_PSEAE: DNA polymerase I (polA) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K02335, DNA polymerase I [EC: 2.7.7.7] (inferred from 94% identity to pba:PSEBR_a40)

Predicted SEED Role

"DNA polymerase I (EC 2.7.7.7)" in subsystem DNA-replication or DNA Repair Base Excision (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166NFF7 at UniProt or InterPro

Protein Sequence (922 amino acids)

>Pf1N1B4_1875 DNA polymerase I (EC 2.7.7.7) (Pseudomonas fluorescens FW300-N1B4)
MSQAPLVLVDGSSYLYRAFHALPPLTTSKGLPTGAVKGVLNMLKSLRKQYPDSPFAVVFD
AKGGTFRDAMFAEYKANRPSMPDDMRVQIEPLHASVKALGFPLLCVDNVEADDVIGTLAR
SSAAADRPVVISTGDKDMAQLVDGHITLVNTMTGSALDVEGVKEKFGVAPEQIIDYLALM
GDSSDNIPGVPGIGPKTASGLLVGVNGGLTELYAQLDIVPTLPIRGAKTLPAKLEEHKEM
AFLSYQLATIKIDVPLDIGLDDLQMGQPDHDKLAELYTLLEFKSWFEENQRDAKRSGQEI
VEVTQEQPGAAEAKYETILDKKHFEAWLDKLDKAPLIAFVTETNGSDAQHAQLVGLSFSV
AANEAAYIPLTHSYMGVPEQLDRDTVLLALKPLLENPNKLKVGQHAKFETNILANCAIGG
DQSNGIIVQGIAYDTMLESYVLDSTATRHDMDSLALKYLGQSKSDIQGIAGKGVKQLTFD
QISLELAGPYAAEDADVTFRLHLALQEKLAATPSLGKVLNEIEMPLMPVLARIERQGALV
DANLLGIQSVELGEKLVALEREAFAIAGEEFNLGSPKQLGVILYEKLGLPVLSKTAKGQA
STAEAVLAELAEQDYPLPKVLMQYRSLSKLKSTYTDRLPEQINTRTGRIHTSYQQAVAAT
GRLSSIDPNLQNIPIRTAEGRRIRQAFIAPKGYKLLAADYSQIELRIMAHLAKDEGLLHA
FRNNLDVHRATAAEVFGVELDAVTNDQRRSAKAINFGLIYGMSAFGLAKQIGVDRKQSQA
YIDRYFARYPGVLEYMERTRAQAAEQGFVETIFGRRLYLPEINAKNQALRKAAERTAINA
PMQGTAADIIKKAMVTVDNWLTSSGLDAKVILQVHDELVLEVREDLIDQVREDIRMHMSS
AAQLDVPLLVEVGVGNNWDEAH