Protein Info for Pf1N1B4_1864 in Pseudomonas fluorescens FW300-N1B4

Annotation: Methionine ABC transporter substrate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 257 signal peptide" amino acids 1 to 19 (19 residues), see Phobius details PF03180: Lipoprotein_9" amino acids 22 to 257 (236 residues), 338 bits, see alignment E=1.1e-105

Best Hits

Swiss-Prot: 44% identical to METQ_PASMU: Probable D-methionine-binding lipoprotein MetQ (metQ) from Pasteurella multocida (strain Pm70)

KEGG orthology group: K02073, D-methionine transport system substrate-binding protein (inferred from 95% identity to pba:PSEBR_a29)

Predicted SEED Role

"Methionine ABC transporter substrate-binding protein" in subsystem Methionine Biosynthesis or Methionine Degradation or Staphylococcal pathogenicity islands SaPI

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166NF78 at UniProt or InterPro

Protein Sequence (257 amino acids)

>Pf1N1B4_1864 Methionine ABC transporter substrate-binding protein (Pseudomonas fluorescens FW300-N1B4)
MKKLLVAFAAVAAFSAHAADTLTVAATPVPHAEILEFVKPALAKEGVDLKVKVFTDYIQP
NVQVAEKRLDANFFQHQPYLDEFNKAKGTNLVSVAGVHLEPLGAYSSKYKALTELPGGAN
VVIPNDATNGGRALLLLAKAGLIKLKDSNNILSTVKDITENTKDLKFRELEAATIPRVLT
QVDLALINTNYALEAKLDPSKDALVIEGSDSPYVNILVARPDDKDSAAMQKLVAALHSPE
VKAFILEKYKGAVLPAF