Protein Info for Pf1N1B4_1817 in Pseudomonas fluorescens FW300-N1B4

Annotation: Glycine cleavage system transcriptional activator

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 299 signal peptide" amino acids 1 to 19 (19 residues), see Phobius details PF00126: HTH_1" amino acids 11 to 66 (56 residues), 67.4 bits, see alignment E=8.8e-23 PF03466: LysR_substrate" amino acids 91 to 292 (202 residues), 121.7 bits, see alignment E=2.9e-39

Best Hits

KEGG orthology group: None (inferred from 94% identity to pfo:Pfl01_0116)

Predicted SEED Role

"Glycine cleavage system transcriptional activator" in subsystem Orphan regulatory proteins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161Z1U8 at UniProt or InterPro

Protein Sequence (299 amino acids)

>Pf1N1B4_1817 Glycine cleavage system transcriptional activator (Pseudomonas fluorescens FW300-N1B4)
MRRKIPSTTALISFEAAARHESFTKAAQELSLTQGAICRQIASLEEFLSVELFRRSRRGV
KLTEAGLSYSRRVATQLDAVERDTLSVMGQQGANMIELAVVPTFGTQWLLPRLKDFQHKH
PEVTVNLTNRTRPFLFADTDFDAAIYFGDADWPGTESHRLMGENPMPVCSPTLLGKKTNL
TPSEIAELPLLQQTTRPYAWRQWFNSQNLNVPRDMTGPRYELFSMLAQAAMHDMGIALIP
PFLIQRELAEKRLVIANAQALSSIKAYYLMIPERKVESASLKAFRDWLVNQAQSYSLEG