Protein Info for Pf1N1B4_180 in Pseudomonas fluorescens FW300-N1B4

Annotation: L-Proline/Glycine betaine transporter ProP

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 459 transmembrane" amino acids 25 to 49 (25 residues), see Phobius details amino acids 60 to 84 (25 residues), see Phobius details amino acids 96 to 114 (19 residues), see Phobius details amino acids 120 to 145 (26 residues), see Phobius details amino acids 163 to 188 (26 residues), see Phobius details amino acids 196 to 217 (22 residues), see Phobius details amino acids 250 to 273 (24 residues), see Phobius details amino acids 285 to 304 (20 residues), see Phobius details amino acids 316 to 334 (19 residues), see Phobius details amino acids 341 to 368 (28 residues), see Phobius details amino acids 381 to 404 (24 residues), see Phobius details amino acids 410 to 428 (19 residues), see Phobius details PF00083: Sugar_tr" amino acids 24 to 231 (208 residues), 83.6 bits, see alignment E=1.4e-27 amino acids 236 to 441 (206 residues), 46 bits, see alignment E=3.8e-16 PF07690: MFS_1" amino acids 55 to 392 (338 residues), 115.9 bits, see alignment E=2e-37 amino acids 298 to 430 (133 residues), 41 bits, see alignment E=1.2e-14

Best Hits

KEGG orthology group: None (inferred from 78% identity to mno:Mnod_7815)

Predicted SEED Role

"L-Proline/Glycine betaine transporter ProP" in subsystem Proline, 4-hydroxyproline uptake and utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161Z397 at UniProt or InterPro

Protein Sequence (459 amino acids)

>Pf1N1B4_180 L-Proline/Glycine betaine transporter ProP (Pseudomonas fluorescens FW300-N1B4)
MSDLSPVEKSGAPPEVAISMKKVAVASVIGTTVEWYDLFVFATASALVFNKVFFPDFDPL
IGTLLAFGTFASAYLARIVGAALFGHFGDRLGRKSMLLVSLLTMGAATFAIGLLPNYAAI
GIWAPILLLLLRVIQGLALGGEWGGAVLMAVEHAPANKRGLYGSWVQIGVPAGTMIANLA
FLVIAAWLSPEDLLAWGWRIPFLASVLLIAVGLYIRLNISETPAFNKVKEAEVQVKMPLA
EVFRKYWKQVVLGGIATMSTGASFNIIVAFGLTYGTQNLGFSRSVMLGVVLLSCAWCIVM
LPVFGALSDRYGRKPIIVAGIVAEALVAFPMFWLMDTKELSLVIFGYLLLMTAFAANYGP
IATFLAELFGTRVRYSGLSISYMLSGLLGSAATPIVTTALLAATGKGSSVAWYMIGAAFI
SLVALLLLTETFKRDISEIQTAVPAADPALGKPIKSAAA