Protein Info for Pf1N1B4_1707 in Pseudomonas fluorescens FW300-N1B4

Annotation: Efflux membrane fusion protein, RND family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 355 signal peptide" amino acids 1 to 19 (19 residues), see Phobius details TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 31 to 349 (319 residues), 230.7 bits, see alignment E=1.1e-72 PF16576: HlyD_D23" amino acids 52 to 257 (206 residues), 49.6 bits, see alignment E=5e-17 PF13533: Biotin_lipoyl_2" amino acids 56 to 101 (46 residues), 33 bits, see alignment 6.1e-12 PF13437: HlyD_3" amino acids 165 to 261 (97 residues), 31.3 bits, see alignment E=4.3e-11

Best Hits

KEGG orthology group: None (inferred from 84% identity to pfo:Pfl01_0231)

Predicted SEED Role

"Efflux membrane fusion protein, RND family"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A162AT83 at UniProt or InterPro

Protein Sequence (355 amino acids)

>Pf1N1B4_1707 Efflux membrane fusion protein, RND family (Pseudomonas fluorescens FW300-N1B4)
MKRLLLLSAGLLLVACSKEEAPPEPVRPVLSVEVKALNQENLGRFAGSIQARYESNVGFR
VPGRIASRNVDVGAEVQKGALLATLDPTDQQNQLRSAQGDLARVQAQLINAQANARRQQE
LFNRGVGAQAQLDIAQTDLKTTQASLDQARAAVDQAQDQLNYVELRADHKAIVTAWNAEA
GQVVTAGQQVVTLAQPDIKEAVIDLPDTLVDQLPADVVFQVASQLDPSITSTAIVREIEP
QAQSATRTRRARLSLTDTPPGFRLGTAISVTLSSAIKPRLELPVSALQEVDGKLRIWVVD
PQTQTVSPREVSLISRTDATVVLANGVKTGERVVSAGVNSLKPGQKVKIDEGSPQ