Protein Info for Pf1N1B4_17 in Pseudomonas fluorescens FW300-N1B4

Annotation: Sensory box/GGDEF family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1085 PF00989: PAS" amino acids 27 to 134 (108 residues), 23.3 bits, see alignment E=1.9e-08 amino acids 152 to 242 (91 residues), 29.4 bits, see alignment E=2.4e-10 amino acids 406 to 452 (47 residues), 22.7 bits, see alignment (E = 2.9e-08) amino acids 531 to 640 (110 residues), 33.5 bits, see alignment E=1.3e-11 TIGR00229: PAS domain S-box protein" amino acids 33 to 146 (114 residues), 34.6 bits, see alignment E=1.9e-12 amino acids 148 to 271 (124 residues), 44.9 bits, see alignment E=1.2e-15 amino acids 273 to 400 (128 residues), 27 bits, see alignment E=4.2e-10 amino acids 402 to 525 (124 residues), 66.2 bits, see alignment E=3.1e-22 amino acids 526 to 649 (124 residues), 77.4 bits, see alignment E=1.1e-25 PF08447: PAS_3" amino acids 46 to 133 (88 residues), 43.1 bits, see alignment E=1.5e-14 amino acids 299 to 386 (88 residues), 40.5 bits, see alignment E=9.7e-14 PF08448: PAS_4" amino acids 47 to 140 (94 residues), 23.4 bits, see alignment E=2e-08 PF13426: PAS_9" amino acids 48 to 137 (90 residues), 11 bits, see alignment E=0.00015 amino acids 161 to 263 (103 residues), 18.5 bits, see alignment E=6.9e-07 amino acids 298 to 392 (95 residues), 12.7 bits, see alignment E=4.7e-05 amino acids 415 to 517 (103 residues), 30.2 bits, see alignment E=1.6e-10 amino acids 540 to 641 (102 residues), 39.1 bits, see alignment E=2.7e-13 PF13188: PAS_8" amino acids 152 to 199 (48 residues), 23.6 bits, see alignment (E = 1.3e-08) amino acids 405 to 459 (55 residues), 26.5 bits, see alignment 1.6e-09 amino acids 530 to 575 (46 residues), 28.1 bits, see alignment (E = 5e-10) TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 651 to 819 (169 residues), 154.6 bits, see alignment E=2e-49 PF00990: GGDEF" amino acids 654 to 815 (162 residues), 164.5 bits, see alignment E=6.3e-52 PF00563: EAL" amino acids 837 to 1074 (238 residues), 252.6 bits, see alignment E=1.2e-78

Best Hits

KEGG orthology group: None (inferred from 94% identity to pfo:Pfl01_1887)

Predicted SEED Role

"Sensory box/GGDEF family protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166MDG5 at UniProt or InterPro

Protein Sequence (1085 amino acids)

>Pf1N1B4_17 Sensory box/GGDEF family protein (Pseudomonas fluorescens FW300-N1B4)
MPPMPRIQALDPKRSEQSWESAPQLLAALNGARLGAWYWDIERGQISWSRGTQALFGFDP
RQPLPEDLEYLDLLPQEDRAKAIRAFHAVIAGAPLEQAMHHRIRWPDGSLHWLEINGSLL
PDKNGRPRMIGVIREITHQRQREQALSSSEKRFATLFHLCPNMVLLTRQEDGLISEANQY
FESLFGWPVQDAIGRTTLELGLWVNPEQRVQLVKATKAKGELINMEVQFRASNGQVHDGI
LSAQKVELEGQPYLLSTFLDTTERKIAEHALKDSQERLDLALDSAQLGTWDWHIPSGMLY
GSARAAQLHGLDPKPFHESFDAFFEGVPDEERGTMRDAYRSLREGPAGNYQLTYRVQLTD
GSSRYLESRARLYRDEQGNPLRMAGTLLDITDQVEREQQLVASEEKFATLFQVSPDPICV
TRQDTGHFIEINSSFTQTFGWSTVDVIGRSADEIGLWDASAKSLQRIERVIREQGLNNVA
IIVRHKDGQSLTCVISSRQISVGDQPCIVTTLRDITQQQRSEAALKASEEKFAKAFHSSP
DAITITERDTGRYLEVNDGFCRLTGYRADEVVGRTVYQVGIWAEEKQRSALLAELQIKGR
VHHQEMLGRNKRGELLTVEVSVEPITLNETACLLLTARDVSLLKNAEAQIRHLAYHDPLT
NLPNRALLMDRLSQQIALLKRHNLRGALMFLDLDHFKHINDSLGHPVGDTVLKIITARLE
ASVRMEDTVARLGGDEFVVLLSGLEGSRSDVSAQVRELADTLRELLSEPMFLDGQRLQVT
PSIGIALIPDHGSTPTDLLKRADIALYRAKDSGRNTTQMYHNTMQKAASERLRMETDLRL
ALSRGEFSVQYQPQVDARNNRITGAEALVRWHHPQLGAQSPTEFIKVLEDSGLILEVGTW
ILDEACAAFKHLIAKGLINPLDFSLCVNISPRQFRQNDFVERIEHSLGSHGLPCSLLKLE
ITEGIVIQNLDDTISKMRRLKKLGVSFAMDDFGTGYSSLTYLKRLPVDTLKIDQSFIRDA
TTDPNDAEIIRAIVAMARSLELEVIAEGVETLEQLAFLQGLGCHLYQGYLHSRPLPVEEF
QKLLK