Protein Info for Pf1N1B4_1681 in Pseudomonas fluorescens FW300-N1B4

Annotation: Peptidase M50

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 698 transmembrane" amino acids 108 to 125 (18 residues), see Phobius details amino acids 145 to 162 (18 residues), see Phobius details amino acids 169 to 191 (23 residues), see Phobius details amino acids 210 to 229 (20 residues), see Phobius details amino acids 241 to 263 (23 residues), see Phobius details amino acids 272 to 291 (20 residues), see Phobius details amino acids 346 to 369 (24 residues), see Phobius details amino acids 375 to 394 (20 residues), see Phobius details amino acids 412 to 430 (19 residues), see Phobius details PF02163: Peptidase_M50" amino acids 183 to 262 (80 residues), 32.4 bits, see alignment E=9.2e-12 PF13533: Biotin_lipoyl_2" amino acids 448 to 492 (45 residues), 41.1 bits, see alignment 1.8e-14

Best Hits

KEGG orthology group: None (inferred from 89% identity to pfo:Pfl01_0147)

Predicted SEED Role

"Peptidase M50"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A162BKS9 at UniProt or InterPro

Protein Sequence (698 amino acids)

>Pf1N1B4_1681 Peptidase M50 (Pseudomonas fluorescens FW300-N1B4)
MSLPSLRADLQLSAAAPALDGSPRWTLADPVRGRYFKLGAAAMRLLRHWSLGDPEHVLRA
ANREPGLPLDGAALEQLLEFLRGHDLISALDPSQRASYSLKAAAQRQSLWQILLHQYLFF
RIPLWRPDAFLNRAWPWLERFGPRALRYGLPATLGLGVFLVSRDWQRFIATFPHLFSLGG
ALAFAVALFFAKLCHEFGHAFMAKRAGCRVQSMGVAFMVLLPMFYTDVSDAWRVNDRRAR
LLIGAGGVLAELVLACIALLAWSLLPDGPGRTAAFMLASATWITTLVINLNPFMRFDGYF
LLSDFWEVDNLQGRAFALCRWRLREFLFGYAAPAPEPWSPKMQRRLLIWGYGAWLWRAVL
FFGIALAVYHLFFKVLGIFLMLVELVWFIFLPILSEWRQWWSRREQAHAPRVLLSGLALL
GLLLLLALPWRSAVELPTMLEAGRASALHAPVAARVKTVNVHDGQIVAQGEVLIELESPD
LDSRQAIVRREIQIQQLQMRRQASRSETAADAGIVEQRLAEAVAEYRGLVAQRERLLLRA
PHGGKVRDLLPQLTVGRWLSTKDPLARVVEDGARLRGYLAEAELWRVAPDASGRFIADDP
MHPAIAVQLSEIDTNGVAYVDQEALTSDHHGPIAVRRDQHQRAEPVQAQYGARLSILENT
PTPVQPLRGIVVLQGSGESLLGVAWRRLAALGVRESGF