Protein Info for Pf1N1B4_1639 in Pseudomonas fluorescens FW300-N1B4

Annotation: transport permease protein of gamma-aminobutyrate

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 464 transmembrane" amino acids 20 to 41 (22 residues), see Phobius details amino acids 47 to 66 (20 residues), see Phobius details amino acids 87 to 107 (21 residues), see Phobius details amino acids 125 to 145 (21 residues), see Phobius details amino acids 155 to 179 (25 residues), see Phobius details amino acids 200 to 222 (23 residues), see Phobius details amino acids 242 to 263 (22 residues), see Phobius details amino acids 288 to 309 (22 residues), see Phobius details amino acids 336 to 355 (20 residues), see Phobius details amino acids 361 to 381 (21 residues), see Phobius details amino acids 402 to 424 (23 residues), see Phobius details amino acids 430 to 450 (21 residues), see Phobius details TIGR01773: GABA permease" amino acids 3 to 453 (451 residues), 738 bits, see alignment E=1.8e-226 PF03845: Spore_permease" amino acids 16 to 259 (244 residues), 30.1 bits, see alignment E=3.4e-11 PF00324: AA_permease" amino acids 19 to 440 (422 residues), 410.6 bits, see alignment E=1.3e-126 PF13520: AA_permease_2" amino acids 20 to 421 (402 residues), 124.9 bits, see alignment E=6.5e-40

Best Hits

Swiss-Prot: 73% identical to BAUD_PSEAE: Probable GABA permease (bauD) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K03293, amino acid transporter, AAT family (inferred from 96% identity to pba:PSEBR_a6)

MetaCyc: 67% identical to 4-aminobutanoate:H+ symporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-384; TRANS-RXN-57

Predicted SEED Role

"transport permease protein of gamma-aminobutyrate"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A162BKQ0 at UniProt or InterPro

Protein Sequence (464 amino acids)

>Pf1N1B4_1639 transport permease protein of gamma-aminobutyrate (Pseudomonas fluorescens FW300-N1B4)
MSSSQSSSNGLEQGLKPRHVTMLSIAGVIGAGLFVGSGHAIAAAGPAVLLAYAAAGALVV
LVMRMLGEMAVASPDTGSFSTYADRAIGHWAGFTIGWLYWWFWVLVIPLEANAAATILHA
WFPNVAIWAFTLVITLLLTATNLFSVKNYGEFEFWFALVKVIAIIGFIGLGILAIFGFLP
TSQVSGVSHLFDTQGFLPNGMGAVLGAILTTMFSFMGTEIVTIAAAESKNPGQQISKATN
SVIWRIGLFYLVSIFIVVALVPWNDPILASVGSYQTVLERMGIPNAKLIVDIVVLVAVTS
CLNSALYTASRMMFSLGKRGDAPAVSQRTNSSGTPYWAVMLSTAAAFLAVFANYVAPAAV
FEFLLASSGAIALLVYLVIAISQLRMRKQRMARGEKIAFSMWLFPGLTYAVIVFIVAALT
IMLFQEAHRVEILATGLLSALVVAAGLLVARRRRNEKLTAAIAR