Protein Info for Pf1N1B4_159 in Pseudomonas fluorescens FW300-N1B4

Annotation: Putative threonine efflux protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 209 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details transmembrane" amino acids 42 to 68 (27 residues), see Phobius details amino acids 75 to 92 (18 residues), see Phobius details amino acids 112 to 133 (22 residues), see Phobius details amino acids 145 to 166 (22 residues), see Phobius details amino acids 186 to 204 (19 residues), see Phobius details PF01810: LysE" amino acids 17 to 205 (189 residues), 115.6 bits, see alignment E=1.1e-37

Best Hits

Swiss-Prot: 35% identical to LEUE_KLEP7: Leucine efflux protein (leuE) from Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)

KEGG orthology group: None (inferred from 70% identity to pfl:PFL_4111)

Predicted SEED Role

"Putative threonine efflux protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166MGK2 at UniProt or InterPro

Protein Sequence (209 amino acids)

>Pf1N1B4_159 Putative threonine efflux protein (Pseudomonas fluorescens FW300-N1B4)
VIDPATLLIFVGAVMLLLLSPGPNMAFVISHGASYGWRGGVASGLGIGLADLILTALTAM
GVTALVASWPPAFDLIRYSGGLYLLWLAFKALQKPSASGTSDVAQVPLKTLLIRAMLNSL
LNPKALLFFMVFLPQFVNAQRGSIAVQLVLLGMVLTLVSGVFHTLLGVFGSTVSRLVSST
STFAKWQSLGLAAVLLLLAVRLVLMTRPA