Protein Info for Pf1N1B4_1550 in Pseudomonas fluorescens FW300-N1B4

Annotation: Arginyl-tRNA synthetase (EC 6.1.1.19)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 578 PF03485: Arg_tRNA_synt_N" amino acids 4 to 90 (87 residues), 86.8 bits, see alignment E=1.9e-28 TIGR00456: arginine--tRNA ligase" amino acids 6 to 578 (573 residues), 548 bits, see alignment E=1.1e-168 PF00750: tRNA-synt_1d" amino acids 101 to 448 (348 residues), 424.3 bits, see alignment E=5.3e-131 PF05746: DALR_1" amino acids 462 to 578 (117 residues), 115.2 bits, see alignment E=2.7e-37

Best Hits

Swiss-Prot: 93% identical to SYR_PSEPF: Arginine--tRNA ligase (argS) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K01887, arginyl-tRNA synthetase [EC: 6.1.1.19] (inferred from 93% identity to pfo:Pfl01_0399)

Predicted SEED Role

"Arginyl-tRNA synthetase (EC 6.1.1.19)" (EC 6.1.1.19)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.19

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166N8Z7 at UniProt or InterPro

Protein Sequence (578 amino acids)

>Pf1N1B4_1550 Arginyl-tRNA synthetase (EC 6.1.1.19) (Pseudomonas fluorescens FW300-N1B4)
MKDTIRQLIQQAITQLVNEGVLPEGLSPAIQVENSRDKKNGDFASNIAMMLAKPAGMKPR
DLAEKIIAALPADENVSKAEIAGPGFLNFFQNTQALATRLDEALADDHIGVRKAGPVQRT
VVDLSAPNLAKEMHVGHLRSTIIGDGVARVLEFLGDEVIRQNHVGDWGTQFGMLMAYLQE
NPITSDELSDLENFYRAAKQRFDESAEFADRARGLVVKLQAGDPDCLALWTKFKDISLSH
CQKIYELLNVKLTMADVMGESAYNDDLINVVNDLKAAGLLVESNGAQCVFLDEFKNADGD
PLPVIIVKADGGYLYATTDLAAVRYRSGVLKADRALYFVDQRQALHFQQVFQVARLAGFV
THPMEMEHMGFGTMNGADGRPFKTRDGGTVKLIDLLTEAQERAYTLVKEKNPELAEDELR
NIAKVVGIGAVKYADLSKHRTSDYSFNFDLMLNFEGNTAPYLLYAYTRVAGVFRKLGKDF
SEVEGQIALEAPHEHELAAKLAQFGEILNNVSDKGTPHILCTYLYDVAGLFSSFYENCPI
LGADTPAQMQSRLRLAALTGRTLKQGLELLGLETLERM