Protein Info for Pf1N1B4_1549 in Pseudomonas fluorescens FW300-N1B4

Annotation: Helicase PriA essential for oriC/DnaA-independent DNA replication

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 142 PF18074: PriA_C" amino acids 39 to 138 (100 residues), 92.3 bits, see alignment E=1.4e-30

Best Hits

Predicted SEED Role

"Helicase PriA essential for oriC/DnaA-independent DNA replication" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase or DNA-replication

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (142 amino acids)

>Pf1N1B4_1549 Helicase PriA essential for oriC/DnaA-independent DNA replication (Pseudomonas fluorescens FW300-N1B4)
VIIQTHLADHPLLVQLTEQGYFAFAEQALSERRSAGLPPFAHLALLRAEAHKPGQAEGFL
DEACSEAERLLAEQNLTGIELLGPVPAPMERRAGRYRAQLLLQATARAPLHRLLSSWLLV
LEQMPSGRAVRWSLDVDPVDLY