Protein Info for Pf1N1B4_1509 in Pseudomonas fluorescens FW300-N1B4

Annotation: Putative histidine ammonia-lyase protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 34% identical to HUTH_MYXXD: Histidine ammonia-lyase (hutH) from Myxococcus xanthus (strain DK 1622)

KEGG orthology group: K01745, histidine ammonia-lyase [EC: 4.3.1.3] (inferred from 95% identity to pfs:PFLU0430)

Predicted SEED Role

"Putative histidine ammonia-lyase protein"

MetaCyc Pathways

L-histidine degradation II (5/5 steps found)
L-histidine degradation VI (7/8 steps found)
L-histidine degradation I (4/4 steps found)
L-histidine degradation III (4/6 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.3.1.3

Use Curated BLAST to search for 4.3.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166N8A3 at UniProt or InterPro

Protein Sequence (514 amino acids)

>Pf1N1B4_1509 Putative histidine ammonia-lyase protein (Pseudomonas fluorescens FW300-N1B4)
MTTPTLEPVTFGELPLRIEDVLALANRQVPTQLQGDPEFRQRIAKGPQFLDSLLDKEGVI
YGVTTGYGDSCVVAVPLHHVEALPRHLYTFHGCGLGKLLDAQATRAVLAARLQSLCQGVS
GVRVELLERLQAFLEYDILPLIPEEGSVGASGDLTPLSYVAATLSGEREVMFRGERRQAA
DVHSELGWEPLVLRPKEALALMNGTAVMTGLACLAYARADYLLQLATRITALNVVALQGN
PEHFDERLFAAKPHPGQMQVAAWLRKDLAIDAPTAPLHRLQDRYSLRCAPHVLGVLADSL
NWLRSFIEIELNSANDNPIIDAEAERVLHGGHFYGGHIAFAMDSLKTLVANVADLLDRQL
ALLVDERYNHGLPSNLSGATADRAMLNHGFKAVQIGTSAWTAEALKNTMPASVFSRSTEC
HNQDKVSMGTISARDAIRVLELTEQVAAATLLAANQGVWLRGQAEDARPLPPALAAMHEE
LAKDFPPVIEDRALEGELRLCLQRIAEQHWRLHA