Protein Info for Pf1N1B4_15 in Pseudomonas fluorescens FW300-N1B4
Annotation: 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 97% identical to LEUC_PSEF5: 3-isopropylmalate dehydratase large subunit (leuC) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)
KEGG orthology group: K01703, 3-isopropylmalate/(R)-2-methylmalate dehydratase large subunit [EC: 4.2.1.33 4.2.1.35] (inferred from 97% identity to pfl:PFL_2063)MetaCyc: 67% identical to 3-isopropylmalate dehydratase subunit LeuC (Escherichia coli K-12 substr. MG1655)
3-isopropylmalate dehydratase. [EC: 4.2.1.33]
Predicted SEED Role
"3-isopropylmalate dehydratase large subunit (EC 4.2.1.33)" in subsystem Branched-Chain Amino Acid Biosynthesis or Leucine Biosynthesis (EC 4.2.1.33)
MetaCyc Pathways
- superpathway of branched chain amino acid biosynthesis (17/17 steps found)
- L-leucine biosynthesis (6/6 steps found)
- 3-methylbutanol biosynthesis (engineered) (6/7 steps found)
- L-isoleucine biosynthesis II (5/8 steps found)
- 2,5-xylenol and 3,5-xylenol degradation (1/13 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from histidine and purine
- C5-Branched dibasic acid metabolism
- Valine, leucine and isoleucine biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 4.2.1.33, 4.2.1.35
Use Curated BLAST to search for 4.2.1.33 or 4.2.1.35
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A162APW5 at UniProt or InterPro
Protein Sequence (472 amino acids)
>Pf1N1B4_15 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33) (Pseudomonas fluorescens FW300-N1B4) MAGKTLYDKLWDSHLVKQRDDGSALIYIDRHIIHEVTSPQAFEGLRLAGRKPWRIDANIA TPDHNVPTTAERKGGIDAIVDQVSRLQVQTLDDNCDEYGIVEFKMNDVRQGIVHVIGPEQ GATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQCLVAKKMKNMLVSVEGQLP FGVTAKDIVLAVIGKIGTAGGNGHAIEFAGSAIRDLSVEGRMTICNMSIEAGARVGLVAA DEKTVAYVKGRPFAPKGAEWDLAVEAWKDLVSDADAKFDTIVELDATQIKPQVSWGTSPE MVLAVDQNVPDPAKEMDLVKRGSIERALKYMGLTANQAITDIQLDRVFIGSCTNSRIEDL RAAAVIAKGRKVASTIKQAIVVPGSGLVKAQAESEGLDKIFLEAGFEWREPGCSMCLAMN PDRLESGEHCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAVNGRFVDVRELI