Protein Info for Pf1N1B4_1497 in Pseudomonas fluorescens FW300-N1B4

Annotation: Potassium efflux system KefA protein / Small-conductance mechanosensitive channel

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1116 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details transmembrane" amino acids 489 to 507 (19 residues), see Phobius details amino acids 536 to 560 (25 residues), see Phobius details amino acids 572 to 596 (25 residues), see Phobius details amino acids 614 to 637 (24 residues), see Phobius details amino acids 643 to 663 (21 residues), see Phobius details amino acids 679 to 700 (22 residues), see Phobius details amino acids 712 to 733 (22 residues), see Phobius details amino acids 776 to 794 (19 residues), see Phobius details amino acids 822 to 843 (22 residues), see Phobius details amino acids 865 to 889 (25 residues), see Phobius details amino acids 897 to 924 (28 residues), see Phobius details PF12795: MscS_porin" amino acids 37 to 270 (234 residues), 185.6 bits, see alignment E=3.1e-58 PF12794: MscS_TM" amino acids 491 to 809 (319 residues), 346.6 bits, see alignment E=3.2e-107 PF21088: MS_channel_1st" amino acids 870 to 911 (42 residues), 37.5 bits, see alignment (E = 4.5e-13) PF00924: MS_channel_2nd" amino acids 913 to 978 (66 residues), 83.2 bits, see alignment 2.8e-27 PF21082: MS_channel_3rd" amino acids 986 to 1069 (84 residues), 61.3 bits, see alignment 2.5e-20

Best Hits

KEGG orthology group: K05802, potassium efflux system protein KefA (inferred from 91% identity to pfo:Pfl01_0445)

Predicted SEED Role

No annotation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A162BKF2 at UniProt or InterPro

Protein Sequence (1116 amino acids)

>Pf1N1B4_1497 Potassium efflux system KefA protein / Small-conductance mechanosensitive channel (Pseudomonas fluorescens FW300-N1B4)
MSTLRTFFIMALLGLSLSISTLQAAEPPSSEAVQASLNKIADRKLPEADQKALQAVLQGT
LTQLNNQRNYDQKLIDLKQQLTNAPKQNIENQRELTRLKATKVVPVAQRYTKETIQQLEQ
LLTERSTQQSDLQKALAEANSLIITAQTRPERAQAEISASQTRIQQINNILKLGKDSGKT
LTAEQRDQLNAELAALNALIPLRRQELAGNSLLQDLGNSQHDLLTEKSNRLDQEIQELQT
LINQKRLAQSQETVTQQSIEAQKAGGSSLLATESAANLKLSDYLLKSTDRLNEVTQQNLQ
TKQQLDSVTQSDSALEEQINVLKGSLLLSKILYKQKQALPRLKLDRDLADQIADIRLYQF
EVSQQRELLSNPTTYVDNLLATQQPEQITPQLRKSLLELATTRADLLERLNRELSAVLNE
SITLQLNQKQLLSTAQSLRATLDEQMFWIPSNKPLDLEWIYAVPERLERQVTTLPWASSV
SELTDGLTQRPLLFLPLAFLIGALLWRRKNLYARLNKVHLDIGHFKRDSQWHTPQAILIN
ILLAMPVSLGLALCGLALQIDARGQNANMGAALLQMAQAWLVFYTAYRILAPGGVAELHF
RWEKPQVEFLQGWIRRLGLVVMALVAVVAVAELQPAILADDVLGMPVVLTCYALMAWLLS
RLLVSSPTHQNASLFRKAVGVLFTALPIALFVAVCFGYYYTALKLSDRLINTLYLLMFWL
VIEATFVRGLSVAARRLAYQRALAKRQAAKEAGDGEAVIEEPTLDIEKVNEQSLRLIRLA
LLGGFIAALYWVWSDLISVFSYLDNITLYEYTSGTGANMSMVPISIGDMLGALIIIGITF
ALARNLPGLLEVFVLSKLNLAQGSSYATTTLLSYVIAGVGFVTTLSTLGVSWDKLQWLVA
ALSVGLGFGMQEIFANFISGIMILFERPVRIGDTITIGNLSGTVSKIRIRATTITDFDRK
DIIVPNKTFITGQLINWSLTDTITRVTLKLGVDYGSDLDLVKELLLKAARDNPRVLKDPE
PHVYFLNFGESTLDHELRMHVRDLGDRNPVLDEVNRFINREFKKQHINISFRQMEVYLKN
LHGQEYKMVPIEPEAKTIVPVVDSKPRREPPPVELD