Protein Info for Pf1N1B4_1481 in Pseudomonas fluorescens FW300-N1B4

Annotation: Protein involved in catabolism of external DNA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 278 PF04378: RsmJ" amino acids 32 to 276 (245 residues), 330.7 bits, see alignment E=2.5e-103

Best Hits

Swiss-Prot: 46% identical to RLMJ_ECOLI: Ribosomal RNA large subunit methyltransferase J (rlmJ) from Escherichia coli (strain K12)

KEGG orthology group: K07115, (no description) (inferred from 94% identity to pfo:Pfl01_0458)

MetaCyc: 46% identical to 23S rRNA m6A2030 methyltransferase (Escherichia coli K-12 substr. MG1655)
RXN0-6998 [EC: 2.1.1.266]

Predicted SEED Role

"Protein involved in catabolism of external DNA"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.266

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161Z129 at UniProt or InterPro

Protein Sequence (278 amino acids)

>Pf1N1B4_1481 Protein involved in catabolism of external DNA (Pseudomonas fluorescens FW300-N1B4)
MNYRHAFHAGNHADVFKHLTLTRLIALMSRKEQPFAYLDTHAGIGLYDLQGDQASRTGEY
LEGIARLWDQPDLPALTADYMKVLHDMNPDGQLRYYPGSPELARRLTRPQDRLMLNEKHP
EDGLLLKDNMAGDRRVKVHLGEGWHVPRAMLPVQEKRAVMLIDPPFEQLDEMQRCAASLK
EAIGRMRQTVAAIWYPVKDQRMLRRFYQDLAGTGAPKLLRVELLVHPLDTPNSLNGSGLA
IANPPWGLEEELRELLPWLSKKLGQTQGGWQMDWLIAE