Protein Info for Pf1N1B4_147 in Pseudomonas fluorescens FW300-N1B4

Annotation: Pyoverdine sidechain non-ribosomal peptide synthetase PvdJ @ Siderophore biosynthesis non-ribosomal peptide synthetase modules

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 690 PF00501: AMP-binding" amino acids 93 to 432 (340 residues), 267.2 bits, see alignment E=3.3e-83 TIGR01733: amino acid adenylation domain" amino acids 114 to 506 (393 residues), 411.9 bits, see alignment E=1.3e-127 PF13193: AMP-binding_C" amino acids 490 to 565 (76 residues), 47.1 bits, see alignment E=5.6e-16 PF00550: PP-binding" amino acids 594 to 656 (63 residues), 48.2 bits, see alignment E=1.6e-16

Best Hits

Predicted SEED Role

No annotation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (690 amino acids)

>Pf1N1B4_147 Pyoverdine sidechain non-ribosomal peptide synthetase PvdJ @ Siderophore biosynthesis non-ribosomal peptide synthetase modules (Pseudomonas fluorescens FW300-N1B4)
VRQEQTHYPLTLVAVTGRELTLGLSFDQACFDHATIANLSTQLEGLMMQFMAHASQPLGA
LQLLDGAPRQAAVDWGKAAITALDSRNVVERIDAQVQRQPQAAAVLFAEQALDYRTLDAR
ANRLAHKLQALGVGPEVRVGVCMRRTPDMLVGLLAILKAGGAYVPLDPEYPQERLLHMLE
DSCATVLLTEAALQAMLPEALPSQVLLVEEGAEWLAGYPSTAPVSQIVSQNLAYVIYTSG
STGKPKGVAITHANLAALIQWSQGVYCAEQLRGVLASTSICFDLSVWEIFVTLASGGCMV
LADNALALPELPARERVTLINTVPSAIAALQRAGQIPASVTTINLAGEPLKQTLVDTLYA
STSVKQVYDLYGPSEDTTYSTFTLRTANGRANIGRPLDNTVAYLLDSQLQLLPAGLAAEL
YLAGAGVTRGYLLRPGLTAERFVPDPFATNGERLYRTGDLVRQGPGDTIEYIGRADHQVK
IRGFRIELSEIEARLLEQPEVREAVVLAQEGAAGKHLHAYVVASQPGSLSLVESLRAALA
NRLPAHMVPSHLHVIDALPLTPNGKLDRKALMALGGNPTLQRYEAPRTDLQWEVATVWQE
VLEVERVGLTDNFFQLGGHSLLATLVVTRIKERLGDKVPLKELFEADTLQAFCTRIEALR
VDLSPVQDELAKSLEALKRLSLDDLEKLIS