Protein Info for Pf1N1B4_1428 in Pseudomonas fluorescens FW300-N1B4

Annotation: Flagellar motor rotation protein MotB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 342 transmembrane" amino acids 23 to 45 (23 residues), see Phobius details PF13677: MotB_plug" amino acids 11 to 63 (53 residues), 64.8 bits, see alignment 4.1e-22 PF00691: OmpA" amino acids 166 to 256 (91 residues), 49.3 bits, see alignment E=5.2e-17

Best Hits

KEGG orthology group: K02557, chemotaxis protein MotB (inferred from 89% identity to pfo:Pfl01_0513)

Predicted SEED Role

"Flagellar motor rotation protein MotB" in subsystem Flagellar motility or Flagellum

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166N6J4 at UniProt or InterPro

Protein Sequence (342 amino acids)

>Pf1N1B4_1428 Flagellar motor rotation protein MotB (Pseudomonas fluorescens FW300-N1B4)
MENNQPIIIKRVKKYAGGHHGGSWKIAFADFATAMMAFFLVLWLLSTATPEQKIAIAGYF
KDPVGFSESGTPYIIDLGGSPTLAPENTLNPEVKSQPQPDKVTIDSEQVEGMAEQVEKER
LELLLQELQNKVEENPQLQKFKDQILFEITQDGLRIQIVDAENRPMFDSGSARLKPYFED
ILLAMADTIKAVPNKISISGHTDAKPYSGTGDFGNWELSANRANAARRALIAGSYPDAQV
ARVVGYASSALFDRENPFNPVNRRIDIIVLTKKAQRAIEGSQGAEPAPVPTQGQGGPGEV
PAPTADPNALPADKEPLPAHELRERLNLFDDPVPKPGESPKQ