Protein Info for Pf1N1B4_1397 in Pseudomonas fluorescens FW300-N1B4

Annotation: Replicative DNA helicase (EC 3.6.1.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 464 TIGR00665: replicative DNA helicase" amino acids 20 to 455 (436 residues), 576.6 bits, see alignment E=1.6e-177 PF00772: DnaB" amino acids 20 to 120 (101 residues), 125.6 bits, see alignment E=1.6e-40 PF03796: DnaB_C" amino acids 197 to 453 (257 residues), 358.4 bits, see alignment E=3.9e-111 PF13481: AAA_25" amino acids 215 to 376 (162 residues), 37.4 bits, see alignment E=4.3e-13

Best Hits

Swiss-Prot: 62% identical to DNAB_SHIFL: Replicative DNA helicase (dnaB) from Shigella flexneri

KEGG orthology group: K02314, replicative DNA helicase [EC: 3.6.4.12] (inferred from 97% identity to psp:PSPPH_0580)

MetaCyc: 62% identical to replicative DNA helicase (Escherichia coli K-12 substr. MG1655)
RXN0-4261 [EC: 5.6.2.3]

Predicted SEED Role

"Replicative DNA helicase (EC 3.6.1.-)" in subsystem DNA-replication (EC 3.6.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.-, 3.6.4.12

Use Curated BLAST to search for 3.6.1.- or 3.6.4.12 or 5.6.2.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161Z5X0 at UniProt or InterPro

Protein Sequence (464 amino acids)

>Pf1N1B4_1397 Replicative DNA helicase (EC 3.6.1.-) (Pseudomonas fluorescens FW300-N1B4)
MNDISAPEQYDLQTAALKVPPHSIEAEQAVLGGLMLDNNAWERVLDQVSDGDFYRHDHRL
IFRAIAKLADQNSPIDVVTLAEQLDKEGQTSQVGGLGYLGELAKNTPSVANIKAYAQIVR
ARATLRQLIGIATEIADSAFNPEGRTAEEILDEAERQIFQIAEARPKTGGPVSVNDLLTK
AIDRIDTLFNTDNAITGLSTGYTDLDEKTSGLQPADLIIVAGRPSMGKTTFAMNLVENAV
LRSDKCVLVYSLEMPGESLIMRMLSSLGRIDQTKVRAGRLDDDDWPRLTSAVNLLNDRKL
FIDDTAGISPSEMRARTRRLVREHGDIALIMIDYLQLMQIPGSGGDNRTNEISEISRSLK
ALAKEFNCPVVALSQLNRSLEQRPNKRPINSDLRESGAIEQDADVIMFVYRDEVYHPETE
HKGIAEIIIGKQRNGPIGTSRLAFIGKYTRFENLAPGSYNFDDE