Protein Info for Pf1N1B4_134 in Pseudomonas fluorescens FW300-N1B4

Annotation: pyoverdine-specific efflux macA-like protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 386 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 38 to 379 (342 residues), 166.8 bits, see alignment E=3e-53 PF16576: HlyD_D23" amino acids 47 to 264 (218 residues), 65 bits, see alignment E=1.2e-21 PF13533: Biotin_lipoyl_2" amino acids 64 to 115 (52 residues), 30.5 bits, see alignment 5e-11 PF13437: HlyD_3" amino acids 186 to 266 (81 residues), 39.2 bits, see alignment E=2e-13

Best Hits

KEGG orthology group: K13888, macrolide-specific efflux protein MacA (inferred from 83% identity to pba:PSEBR_a3908)

Predicted SEED Role

"pyoverdine-specific efflux macA-like protein" in subsystem Siderophore Pyoverdine

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166MG29 at UniProt or InterPro

Protein Sequence (386 amino acids)

>Pf1N1B4_134 pyoverdine-specific efflux macA-like protein (Pseudomonas fluorescens FW300-N1B4)
MKRPRPTRRALFVALCLIPAAALAAWQVIPPGREKLATATVTRADIENSVTALGTLQPRR
YVDVGAQASGQIQKIHVEAGDQVKEGQLLVEIDPSTQKAKLDAGRFSIDNLKAQLQEQRA
QHDLAQQKYQRQQNLKAGGATREEDVQTARAEVRARQARIDMFQAQIRQAQASLRSDEAE
LGYTRIYAPMSGTVVAVGAREGQTLNAQQQTPLILRIAKLSPMTVWAEVSEADIGQVKPG
MTAWFTTLSGGSRRWSSTVRQILPVPPRPLEQSQGSPTSGRSGSERVVLYTVLLDVDNAD
NALMTDMTAQVFFVAQQAQNVLTVPTAALQNGTAADRQVAQVVATNGEVQRREVRTGISD
RLRTQILEGLAEGDHVLSAPLTGSGD