Protein Info for Pf1N1B4_1309 in Pseudomonas fluorescens FW300-N1B4

Annotation: Biotin carboxylase of acetyl-CoA carboxylase (EC 6.3.4.14)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00514: acetyl-CoA carboxylase, biotin carboxylase subunit
  • amino acids 5 to 448 (444 residues), 737.3 bits, E=3.3e-226
• PF00289: Biotin_carb_N
  • amino acids 5 to 113 (109 residues), 153.6 bits, E=6.5e-49
• PF02786: CPSase_L_D2
  • amino acids 118 to 325 (208 residues), 266.4 bits, E=4.2e-83
• PF07478: Dala_Dala_lig_C
  • amino acids 146 to 294 (149 residues), 26.8 bits, E=8.6e-10
• PF02785: Biotin_carb_C
  • amino acids 339 to 444 (106 residues), 134.3 bits, E=4.7e-43

Best Hits

Swiss-Prot: 87% identical to ACCC_PSEAE: Biotin carboxylase (accC) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K01961, acetyl-CoA carboxylase, biotin carboxylase subunit [EC: 6.3.4.14 6.4.1.2] (inferred from 98% identity to pba:PSEBR_a633)

MetaCyc: 70% identical to biotin carboxylase (Escherichia coli K-12 substr. MG1655)
Biotin carboxylase. [EC: 6.3.4.14]

Predicted SEED Role

"Biotin carboxylase of acetyl-CoA carboxylase (EC 6.3.4.14)" in subsystem Fatty Acid Biosynthesis FASII (EC 6.3.4.14)

MetaCyc Pathways

• biotin-carboxyl carrier protein assembly (4/4 steps found)
• fatty acid biosynthesis initiation (type I) (1/3 steps found)
• 3-hydroxypropanoate cycle (6/13 steps found)
• jadomycin biosynthesis (3/9 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (9/18 steps found)
• glyoxylate assimilation (5/13 steps found)
• superpathway of the 3-hydroxypropanoate cycle (6/18 steps found)
• superpathway of mycolate biosynthesis (21/239 steps found)

KEGG Metabolic Maps

• Fatty acid biosynthesis
• Propanoate metabolism
• Pyruvate metabolism
• Tetracycline biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 6.3.4.14, 6.4.1.2

Use Curated BLAST to search for 6.3.4.14 or 6.4.1.2

Sequence Analysis Tools

See A0A161Z5Q7 at UniProt or InterPro

Protein Sequence (452 amino acids)

>Pf1N1B4_1309 Biotin carboxylase of acetyl-CoA carboxylase (EC 6.3.4.14) (Pseudomonas fluorescens FW300-N1B4)
MTAKLEKVLIANRGEIALRILRACKEMGIKTVAVYSKADKELMHLGLADESVCIGPASAA
HSYLHIPAIIAAAEVTGATAIHPGYGFLAENADFAEQVENSGFAFIGPKAETIRLMGDKV
SAKHAMIAAGVPTVPGSDGPLPEDEETALGIGREVGYPVIIKAAGGGGGRGMRVVHKEED
LISSAKLTRSEAGAAFGNPMVYLEKFLTNPRHVEVQVLSDGQGHAIHLGDRDCSLQRRHQ
KVLEEAPAPGIDEKARAEVLARCVKACIDIGYRGAGTFEFLYENGHFYFIEMNTRVQVEH
PVSEMVTGIDIVKEMLSIAAGNKLSYTQDDVVIRGHALECRINAEDPKTFMPSPGTVKHF
HAPGGNGVRVDSHLYSGYAVPPNYDSLIGKLITYGATRDEAMARMRNALDEIVVDGIKTN
IPLHRDLVRDAGFCEGGVNIHYLEHKLAGEKH