Protein Info for Pf1N1B4_128 in Pseudomonas fluorescens FW300-N1B4

Annotation: Methylglyoxal reductase, acetol producing (EC 1.1.1.-) / 2,5-diketo-D-gluconic acid reductase B (EC 1.1.1.274)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00248: Aldo_ket_red
  • amino acids 8 to 251 (244 residues), 178.1 bits, E=1.1e-56

Best Hits

Swiss-Prot: 71% identical to DKGB_YERPE: 2,5-diketo-D-gluconic acid reductase B (dkgB) from Yersinia pestis

KEGG orthology group: K06222, 2,5-diketo-D-gluconate reductase B [EC: 1.1.1.274] (inferred from 91% identity to pfo:Pfl01_1865)

MetaCyc: 70% identical to methylglyoxal reductase DkgB (Escherichia coli K-12 substr. MG1655)
Aryl-alcohol dehydrogenase (NADP(+)). [EC: 1.1.1.91]; RXN0-7020 [EC: 1.1.1.91, 1.1.1.346]; Aldehyde reductase. [EC: 1.1.1.91, 1.1.1.346, 1.1.1.21]

Predicted SEED Role

"Methylglyoxal reductase, acetol producing (EC 1.1.1.-) / 2,5-diketo-D-gluconic acid reductase B (EC 1.1.1.274)" in subsystem D-gluconate and ketogluconates metabolism (EC 1.1.1.-, EC 1.1.1.274)

MetaCyc Pathways

• methylglyoxal degradation III (1/2 steps found)
• L-ascorbate biosynthesis VIII (engineered pathway) (4/7 steps found)
• ketogluconate metabolism (4/8 steps found)
• superpathway of methylglyoxal degradation (4/8 steps found)
• detoxification of reactive carbonyls in chloroplasts (4/10 steps found)
• superpathway of pentose and pentitol degradation (11/42 steps found)

KEGG Metabolic Maps

• Alkaloid biosynthesis I
• Aminosugars metabolism
• Ascorbate and aldarate metabolism
• Benzoate degradation via CoA ligation
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of type II polyketide products
• Biosynthesis of unsaturated fatty acids
• Bisphenol A degradation
• Butanoate metabolism
• C21-Steroid hormone metabolism
• Fructose and mannose metabolism
• Galactose metabolism
• Glycerolipid metabolism
• Glycine, serine and threonine metabolism
• Insect hormone biosynthesis
• Linoleic acid metabolism
• Nucleotide sugars metabolism
• Pentose and glucuronate interconversions
• Polyketide sugar unit biosynthesis
• Pyruvate metabolism
• Retinol metabolism
• Tetrachloroethene degradation

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.-

Use Curated BLAST to search for 1.1.1.- or 1.1.1.21 or 1.1.1.274 or 1.1.1.346 or 1.1.1.91

Sequence Analysis Tools

See A0A162BH55 at UniProt or InterPro

Protein Sequence (267 amino acids)

>Pf1N1B4_128 Methylglyoxal reductase, acetol producing (EC 1.1.1.-) / 2,5-diketo-D-gluconic acid reductase B (EC 1.1.1.274) (Pseudomonas fluorescens FW300-N1B4)
MSIPAFGLGTFRLQGQVVIDSVSTGLELGYRVIDTAQIYKNEAEVGQAIAASGIARDELF
ITSKIWVANFAKDRLIDSLKESLNKLQTDYLDLTLIHWPSPEDQVPVEEFMGALLQAKQL
GLTRKIGVSNFTVDLMQQAITAIGAENIATNQIELHPYLQNRKVVEFAQSQGIRITSYMT
LAYGEVLKDPLIQQIADRLQATPAQVTLAWAMQLGYAVIPSSTKRANLQGNLQACALTLS
DADMALIAALDRGQRLTSPKGIAPQWD