Protein Info for Pf1N1B4_1259 in Pseudomonas fluorescens FW300-N1B4

Annotation: Multidrug resistance transporter, Bcr/CflA family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 398 signal peptide" amino acids 1 to 34 (34 residues), see Phobius details transmembrane" amino acids 43 to 61 (19 residues), see Phobius details amino acids 71 to 90 (20 residues), see Phobius details amino acids 96 to 117 (22 residues), see Phobius details amino acids 129 to 151 (23 residues), see Phobius details amino acids 158 to 179 (22 residues), see Phobius details amino acids 207 to 228 (22 residues), see Phobius details amino acids 244 to 263 (20 residues), see Phobius details amino acids 274 to 295 (22 residues), see Phobius details amino acids 301 to 324 (24 residues), see Phobius details amino acids 337 to 358 (22 residues), see Phobius details amino acids 364 to 383 (20 residues), see Phobius details TIGR00710: drug resistance transporter, Bcr/CflA subfamily" amino acids 3 to 379 (377 residues), 287 bits, see alignment E=1.5e-89 PF07690: MFS_1" amino acids 15 to 348 (334 residues), 130.2 bits, see alignment E=9.1e-42 PF00083: Sugar_tr" amino acids 40 to 182 (143 residues), 38 bits, see alignment E=9.6e-14

Best Hits

KEGG orthology group: K07552, MFS transporter, DHA1 family, bicyclomycin/chloramphenicol resistance protein (inferred from 89% identity to pfo:Pfl01_0664)

Predicted SEED Role

"Multidrug resistance transporter, Bcr/CflA family" in subsystem Copper homeostasis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A162ASH9 at UniProt or InterPro

Protein Sequence (398 amino acids)

>Pf1N1B4_1259 Multidrug resistance transporter, Bcr/CflA family (Pseudomonas fluorescens FW300-N1B4)
MNFRTILILGALSAFGPLAIDFYLPAFPAMALAFGTDEKHVQLTLAAYFLGLSIGQLAYG
PVADRFGRRIPLLTGVGLFTAASLACAYAPTLEWLIGARFVQALGGCAGMVIARAVVSDK
CDAVGSAKVFSQLMLVMGLAPILAPMLGGLLVNTTGWQSIFLALTGFSALAGLAVALGLP
ESLPAHVPRQPLSGALRQYGRLLADPIFLGHALTGGVAIAGMFAYIAGSPFVFIKLYGVP
AEHFGWLFGINAAGFILVAQVNARMLAKRGPAFLLARTVWFYLSGGLALLAVSALHTEQL
WPLLIPLFLCIASLGCIVPNASACAMNGQGARAGSASAMLGCLQFSVAAGAAALVGVLHD
GSAMPMAMVISLCGILVVSAATLTRRLQNARALTQAQV