Protein Info for Pf1N1B4_125 in Pseudomonas fluorescens FW300-N1B4

Annotation: Nicotinamidase family protein YcaC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 208 transmembrane" amino acids 108 to 127 (20 residues), see Phobius details PF00857: Isochorismatase" amino acids 14 to 165 (152 residues), 81.8 bits, see alignment E=3.5e-27

Best Hits

Swiss-Prot: 88% identical to YCAC_ECOLI: Probable hydrolase YcaC (ycaC) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 97% identity to pba:PSEBR_a462)

Predicted SEED Role

"Nicotinamidase family protein YcaC" in subsystem NAD and NADP cofactor biosynthesis global

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166MFU2 at UniProt or InterPro

Protein Sequence (208 amino acids)

>Pf1N1B4_125 Nicotinamidase family protein YcaC (Pseudomonas fluorescens FW300-N1B4)
MTTSYKRLDKDNAAVLLVDHQAGLLSLVRDIDPDRFKNNVLALADLAKYFKLPTILTTSF
ETGPNGPLVPELKALFPDAPYIARPGQINAWDNEDFVKAIKATGKKQLIIAGVVTEVCVA
FPALSALAEGFEVFVVTDASGTFNELTRQSAWDRMSSSGAQLMTWFGLACELHRDWRNDV
EGLATLFSNHIPDYRNLMTSYNTLTNSK