Protein Info for Pf1N1B4_1235 in Pseudomonas fluorescens FW300-N1B4

Annotation: Exodeoxyribonuclease V beta chain (EC 3.1.11.5)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1229 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details TIGR00609: exodeoxyribonuclease V, beta subunit" amino acids 9 to 1211 (1203 residues), 1085.7 bits, see alignment E=0 PF00580: UvrD-helicase" amino acids 11 to 158 (148 residues), 78.5 bits, see alignment E=1.8e-25 amino acids 337 to 445 (109 residues), 90.1 bits, see alignment E=5e-29 PF13245: AAA_19" amino acids 18 to 433 (416 residues), 42.8 bits, see alignment E=1.4e-14 PF13361: UvrD_C" amino acids 702 to 841 (140 residues), 55.6 bits, see alignment E=1.5e-18 PF12705: PDDEXK_1" amino acids 988 to 1194 (207 residues), 25.7 bits, see alignment E=2.8e-09

Best Hits

KEGG orthology group: K03582, exodeoxyribonuclease V beta subunit [EC: 3.1.11.5] (inferred from 82% identity to pfs:PFLU5213)

Predicted SEED Role

"Exodeoxyribonuclease V beta chain (EC 3.1.11.5)" in subsystem DNA-replication (EC 3.1.11.5)

Isozymes

Compare fitness of predicted isozymes for: 3.1.11.5

Use Curated BLAST to search for 3.1.11.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166N2V2 at UniProt or InterPro

Protein Sequence (1229 amino acids)

>Pf1N1B4_1235 Exodeoxyribonuclease V beta chain (EC 3.1.11.5) (Pseudomonas fluorescens FW300-N1B4)
MTTKTPLALAFPLCGSQLIEASAGTGKTFTISALYLRLVLGHGGESSGFGRELLPPQILV
VTFTDAATKELRERIRTRLAEAARFFRDETSAPDGLIAELRDQYLPEQWSGCANRLDIAA
QWMDEAAVSTIHSWCQRMLREHAFDSGSLFTQTLETDHSDLLGEVLRDYWRLFCYPMQGD
ALNWVRGNWGGPAALLPRVRGLFASERDSVEGKEPAELIAECLQQRRAALLELKMPWRQW
ADELLAICHQGVASKSVDGRKMQPRYFEPWFEKLRAWAEDESLELLDIGTGFTRLTPDGM
SEAWKGEAPRHPGLDAMAGLKTSLDGLPTPDAAVLQHAAHWVGARFEEEKRRRAEMGFDD
MLLRLDAALQSEGGERLATLIREQFPVALIDEFQDTDPVQYRIFESIYRIEDNNPETGLF
LIGDPKQAIYAFRGADIYTYLRARQATTGRLHTLGTNFRSSHGMVSAVNHVFERAESREQ
GRGAFLFREKNGENPVPFLPVESQGRKEVLQIDAQAVPALNIWHLSADQPLSGAVYRQQL
AAACASEITALLNGGQQGCAGFIQDGKDFKGLLPSDIAILVRDGKEAQAVRGELSARGVR
SVYLSDKDSVFAAQEAHDLLTWLKACAEPDVERPLRAALACITLNLSLAELERLNQDELA
WETRVMQFRGYRELWRKQGVLPMLRRLLHDFRLPQALIARNDGERVLTNLLHLSELLQQA
AAELDGEQALIRHLSEHLALSGQAGEEQILRLESDEQLVKVVTIHKSKGLEYPLVFLPFI
CSAKPVDGSRLPLYYHDATGKAQVSLKPTPELIAQADDERLAEDLRLLYVALTRAQHACW
LGVTDLKRGNNNGSTLHLSALGYLLGGGAPLAESAGLRRWLEDLQQDCPALNYGEMPEAS
AEHYHPPRNEATLLAPLIPKRKASENWWIASYSALRIGDSLSVGSDEAPESPQAQKLFDD
ERLDPQAPREVIAGGADIHRFPRGPNPGTFLHGLLEWAGDEGFAAAPQTVEDAIARRCNR
RGWEGWITTLSDWLQHLLKSPLHIGGGQAPVVFGQLTQYRVEMEFWFASHKVDVLKLDEL
VRQYTHNGVARVAAEPVLLNGMFKGFIDLTFEHDGRYYVADYKSNWLGVDDAAYTVPAME
QSILDNRYDLQYVLYLLALHRQLKARLVDYDYERHVGGALYLFLRGTRAASQGVYFARPP
RELIERLDRLFQGKPEPKAEPAWEQGVLL