Protein Info for Pf1N1B4_1218 in Pseudomonas fluorescens FW300-N1B4

Annotation: Probable Co/Zn/Cd efflux system membrane fusion protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 367 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 46 to 351 (306 residues), 243.1 bits, see alignment E=1.8e-76 PF16576: HlyD_D23" amino acids 65 to 268 (204 residues), 80.3 bits, see alignment E=1.9e-26 PF13533: Biotin_lipoyl_2" amino acids 69 to 109 (41 residues), 30.9 bits, see alignment 2.7e-11 PF13437: HlyD_3" amino acids 166 to 265 (100 residues), 49.9 bits, see alignment E=7.1e-17

Best Hits

KEGG orthology group: None (inferred from 89% identity to pfo:Pfl01_0706)

Predicted SEED Role

"Probable Co/Zn/Cd efflux system membrane fusion protein" in subsystem Cobalt-zinc-cadmium resistance

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166N2G2 at UniProt or InterPro

Protein Sequence (367 amino acids)

>Pf1N1B4_1218 Probable Co/Zn/Cd efflux system membrane fusion protein (Pseudomonas fluorescens FW300-N1B4)
MLARNKVKSLVFVLLVIIIGSAFGWRFWWPAPVSAEQRIPSTKVGLAQVKKQPYTYYLEA
IGKLEAQRQVLVSAEVSGKVVDIDFESGDQVAAGQVLLTLNDAPEQGELVRLKGEFESAK
AQFARVQELAKTGAESRRAFDSARAQYDAAKGDMERMQAQIAQRHIRAPFAGTLGIRQAH
LGQYLEAGGAVATLTDPGVLRVNFTLAERDGSRVQLGQTVQAKVDAWADVKFQGKIVAVD
PQINQSHTINVQAVITDTKKLLRPGMYARVSVTLPQTSELVIPETAITYNAYGESVFSVY
TDDLGVQKVKRESIKVGERRDGWAVVDKGLNEGVQVVTSGQLKLHDGVAVEGVPDTVGLN
LTTGLEK