Protein Info for Pf1N1B4_1172 in Pseudomonas fluorescens FW300-N1B4

Annotation: Carbamoyl-phosphate synthase large chain (EC 6.3.5.5)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01369: carbamoyl-phosphate synthase, large subunit
  • amino acids 2 to 1053 (1052 residues), 1574.7 bits, E=0
• PF02786: CPSase_L_D2
  • amino acids 128 to 334 (207 residues), 243.3 bits, E=9.6e-76
  • amino acids 673 to 875 (203 residues), 121.1 bits, E=2.4e-38
• PF02787: CPSase_L_D3
  • amino acids 427 to 505 (79 residues), 84.3 bits, 2.5e-27
• PF02222: ATP-grasp
  • amino acids 682 to 845 (164 residues), 43.2 bits, E=1.7e-14
• PF02655: ATP-grasp_3
  • amino acids 682 to 845 (164 residues), 33.5 bits, E=2.1e-11
• PF07478: Dala_Dala_lig_C
  • amino acids 698 to 843 (146 residues), 28.2 bits, E=6.5e-10
• PF02142: MGS
  • amino acids 959 to 1041 (83 residues), 79 bits, 1.2e-25

Best Hits

Swiss-Prot: 67% identical to CARB_BUCAI: Carbamoyl-phosphate synthase large chain (carB) from Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS)

KEGG orthology group: K01955, carbamoyl-phosphate synthase large subunit [EC: 6.3.5.5] (inferred from 78% identity to abo:ABO_0318)

MetaCyc: 75% identical to carbamoyl-phosphate synthetase large subunit (Escherichia coli K-12 substr. MG1655)
Carbamoyl-phosphate synthase (glutamine-hydrolyzing). [EC: 6.3.5.5]

Predicted SEED Role

"Carbamoyl-phosphate synthase large chain (EC 6.3.5.5)" in subsystem De Novo Pyrimidine Synthesis or Macromolecular synthesis operon (EC 6.3.5.5)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (42/46 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
• L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (9/9 steps found)
• superpathway of arginine and polyamine biosynthesis (14/17 steps found)
• UMP biosynthesis I (6/6 steps found)
• UMP biosynthesis II (6/6 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (8/9 steps found)
• L-citrulline biosynthesis (7/8 steps found)
• L-arginine degradation V (arginine deiminase pathway) (4/4 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• L-citrulline degradation (3/3 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• UMP biosynthesis III (5/6 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• superpathway of L-citrulline metabolism (9/12 steps found)
• L-glutamine degradation I (1/1 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (3/4 steps found)
• L-glutamate and L-glutamine biosynthesis (5/7 steps found)
• L-arginine biosynthesis IV (archaea) (4/9 steps found)
• allantoin degradation IV (anaerobic) (2/9 steps found)
• guadinomine B biosynthesis (2/13 steps found)

KEGG Metabolic Maps

• Glutamate metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.5

Use Curated BLAST to search for 6.3.5.5

Sequence Analysis Tools

See A0A166N1I5 at UniProt or InterPro

Protein Sequence (1073 amino acids)

>Pf1N1B4_1172 Carbamoyl-phosphate synthase large chain (EC 6.3.5.5) (Pseudomonas fluorescens FW300-N1B4)
MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRVILVNSNPATIMTDPAM
ADATYIEPIKWQTVAKIIEKERPDALLPTMGGQTALNCALDLEREGVLEKFGVEMIGANA
DTIDKAEDRSRFDKAMKSIGLDCPRSGIAHSMEEANAVLERLGFPCIIRPSFTMGGTGGG
IAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEMEVVRDKKDNCIIVCSIENFDPM
GVHTGDSITVAPAQTLTDKEYQILRNASLAVLREIGVETGGSNVQFGICPNTGRMVVIEM
NPRVSRSSALASKATGFPIAKVAAKLAVGYTLDELSNDITGGKTPASFEPSIDYVVTKLP
RFAFEKFPKADARLTTQMKSVGEVMAIGRTFQESLQKALRGLEVGVCGLDEKLDLSNPES
MSILKRELTVPGAERIWYVADAFRAGLSVEDIFGMNMIDPWFLVQIEDLIKEEEKVKTLG
LASIDHDVMFRLKRKGFSDMRLAKLLGVTEKALRRHRHKLEIFPVYKRVDTCAAEFATDT
AYLYSTYEEECEAAPSGRDKIMILGGGPNRIGQGIEFDYCCVHAALALREDGYETIMVNC
NPETVSTDYDTSDRLYFEPVTLEDVLEIVRVEKPKGVIVQYGGQTPLKLARALEEAGVPI
IGTSPDAIDRAEDRERFQQMVERLNLRQPPNATVRSEDEAIRAAAKIGYPLVVRPSYVLG
GRAMEIVYEEEELKRYLRDAVKVSNDSPVLLDHFLNCAIEMDVDAVCDGTDVVIGAIMQH
IEQAGVHSGDSACSLPPYSLPAHIQDEMREQVKKMALELGVVGLMNVQLALQGEDIYVIE
VNPRASRTVPFVSKCIGVSLAMIAARVMAGKTLKEIGFTKEIIPNFYSVKEAVFPFAKFP
GVDPILGPEMKSTGEVMGVGDTFGEAFAKAQMGASEVLPTGGTAFISVRDDDKPLVAGVA
RDLINLGFEVVATAGTAKLIEAAGLKVRRVNKVTEGRPHVVDMIKNDEVTLIINTTEGRQ
SIADSYSIRRNALQHKIYCTTTIAAGEAICEALKFGPEKTVRRLQDLHAGLKA