Protein Info for Pf1N1B4_1164 in Pseudomonas fluorescens FW300-N1B4

Annotation: Triosephosphate isomerase (EC 5.3.1.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00419: triose-phosphate isomerase
  • amino acids 1 to 236 (236 residues), 208.9 bits, E=4.7e-66
• PF00121: TIM
  • amino acids 1 to 244 (244 residues), 297.7 bits, E=3.4e-93

Best Hits

Swiss-Prot: 96% identical to TPIS_PSEPF: Triosephosphate isomerase (tpiA) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K01803, triosephosphate isomerase (TIM) [EC: 5.3.1.1] (inferred from 96% identity to pfo:Pfl01_0775)

MetaCyc: 48% identical to triose-phosphate isomerase (Escherichia coli K-12 substr. MG1655)
Triose-phosphate isomerase. [EC: 5.3.1.1]

Predicted SEED Role

"Triosephosphate isomerase (EC 5.3.1.1)" in subsystem Calvin-Benson cycle or Glycolysis and Gluconeogenesis or Glycolysis and Gluconeogenesis, including Archaeal enzymes or MLST (EC 5.3.1.1)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (21/26 steps found)
• superpathway of glycolysis and the Entner-Doudoroff pathway (14/17 steps found)
• gluconeogenesis I (11/13 steps found)
• formaldehyde assimilation III (dihydroxyacetone cycle) (10/12 steps found)
• homolactic fermentation (10/12 steps found)
• superpathway of anaerobic sucrose degradation (15/19 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (17/22 steps found)
• glycolysis II (from fructose 6-phosphate) (9/11 steps found)
• glycolysis III (from glucose) (9/11 steps found)
• ethene biosynthesis V (engineered) (19/25 steps found)
• glycolysis IV (8/10 steps found)
• Calvin-Benson-Bassham cycle (10/13 steps found)
• glycolysis I (from glucose 6-phosphate) (10/13 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (14/19 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (19/26 steps found)
• glycolysis V (Pyrococcus) (7/10 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (19/27 steps found)
• sucrose degradation V (sucrose α-glucosidase) (3/5 steps found)
• gluconeogenesis III (8/12 steps found)
• glycolysis VI (from fructose) (7/11 steps found)
• oxygenic photosynthesis (11/17 steps found)
• glycerol degradation to butanol (10/16 steps found)
• superpathway of hexitol degradation (bacteria) (11/18 steps found)
• superpathway of N-acetylneuraminate degradation (13/22 steps found)
• gluconeogenesis II (Methanobacterium thermoautotrophicum) (9/18 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (20/56 steps found)

KEGG Metabolic Maps

• Carbon fixation in photosynthetic organisms
• Fructose and mannose metabolism
• Glycolysis / Gluconeogenesis
• Inositol phosphate metabolism

Isozymes

Compare fitness of predicted isozymes for: 5.3.1.1

Use Curated BLAST to search for 5.3.1.1

Sequence Analysis Tools

See A0A162BJI5 at UniProt or InterPro

Protein Sequence (247 amino acids)

>Pf1N1B4_1164 Triosephosphate isomerase (EC 5.3.1.1) (Pseudomonas fluorescens FW300-N1B4)
MVAGNWKMHGTRASVAELINGLGHLALPSGVDVAVFPPCLHINQVIDGLKGKSISVGAQN
SAVESMQGALTGEIAPSQLVDAGCSLVLVGHSERRQIMGEQDGMLIRKFAAAQACGLIPV
LCVGETLEEREAGKTLEVVGRQLGSIIEELGVGVFAKAVIAYEPVWAIGTGLTASPQQAQ
DVHAAIRAQLAAENSEVAQGVRLLYGGSVKAANAVELFGMPDIDGGLIGGASLNADEFGA
ICRAAGN