Protein Info for Pf1N1B4_1146 in Pseudomonas fluorescens FW300-N1B4

Updated annotation (from data): fructose-specific PTS system (fructose 1-phosphate forming), EI, Hpr, and EII-A components
Rationale: Specifically important for utilizing D-Fructose.
Original annotation: Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 953 transmembrane" amino acids 591 to 601 (11 residues), see Phobius details PF00359: PTS_EIIA_2" amino acids 4 to 140 (137 residues), 95.3 bits, see alignment E=9e-31 PF00381: PTS-HPr" amino acids 285 to 366 (82 residues), 59.9 bits, see alignment E=5.3e-20 TIGR01417: phosphoenolpyruvate-protein phosphotransferase" amino acids 403 to 951 (549 residues), 495.7 bits, see alignment E=8.3e-153 PF05524: PEP-utilisers_N" amino acids 404 to 519 (116 residues), 82.5 bits, see alignment E=7e-27 PF00391: PEP-utilizers" amino acids 544 to 616 (73 residues), 78.6 bits, see alignment 5.2e-26 PF02896: PEP-utilizers_C" amino acids 644 to 925 (282 residues), 361.5 bits, see alignment E=7.6e-112

Best Hits

KEGG orthology group: K11189, phosphocarrier protein (inferred from 96% identity to pfo:Pfl01_0793)

Predicted SEED Role

"Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9)" in subsystem Fructose and Mannose Inducible PTS or Fructose utilization or Mannitol Utilization (EC 2.7.3.9)

Isozymes

Compare fitness of predicted isozymes for: 2.7.3.9

Use Curated BLAST to search for 2.7.3.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161Z0A7 at UniProt or InterPro

Protein Sequence (953 amino acids)

>Pf1N1B4_1146 fructose-specific PTS system (fructose 1-phosphate forming), EI, Hpr, and EII-A components (Pseudomonas fluorescens FW300-N1B4)
MLELTIEQISMGQSAVDKATALQLLADRLVTDGLVADGYLAGLQAREAQGSTFLGQGIAI
PHGTPQTRDLVFSTGVRLMQFPDGVDWGDGQIVYLAIGIAAKSDEHLRLLQLLTRALGET
DLGQALRRASTAEALLKLLQGAPQELALDAQMIGLGVSADDFEELVWRGARLLRQADCVS
NGFAGVLQQVDALPLGDGLWWLHSEQTVKRPGLAFVTPDKPMRYLGQPLSGLFCLASLGE
AHQALLERLCALLIEGRGHELGRATSSRAVLEVLGGEVPADWPSARIALANTHGLHARPA
KILAQLAKSFEGEIRVRIVDGHDSAVSVKSLSKLLSLGARRGQVLEFIAEPTIAADALPA
LLAAIEEGLGEEVEPLPAVSQHREVIADVAEVLLAPASGSLIQAIAAAPGIAIGPAHIQV
QQVIDYPLRGESAAIERERLKQALADVRRDIEGLIERSKAKAIREIFITHQEMLDDPELT
DEVDTRLKQGESAEAAWMAVIEAAAKQQESLQDALLAERAADLRDIGRRVLAQLSGVETP
AEPEQPYILVMDEVGPSDVARLDPTRVAGILTARGGATAHSAIVARALGIPALVGAGAAV
LLLKPGTPLLIDGQRGRLHVDADAATLQRATEERDTRELRLKAAAEQRHQPALTTDGHAV
EVFANIGESAGVTSAVEQGAEGIGLLRTELIFMAHSQAPDEATQEVEYRRVLDGLAGRPL
VVRTLDVGGDKPLPYWPIAKEENPFLGVRGIRLTLQRPQVMEAQLRALLRAADNRPLRIM
FPMVGSVDEWRQARDMTERLRLEIPVADLQLGIMIEVPSAALLAPVLAKEVDFFSVGTND
LTQYTLAIDRGHPTLSAQADGLHPAVLQLIDITVRAAHAHGKWVGVCGELAADPLAVPVL
VGLGVDELSVSGRSIAEVKARIRELSLTQTQTLAQQALAVGSANEVRALVEAL