Protein Info for Pf1N1B4_1143 in Pseudomonas fluorescens FW300-N1B4

Annotation: Phosphodiesterase/alkaline phosphatase D

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 513 signal peptide" amino acids 1 to 31 (31 residues), see Phobius details PF16655: PhoD_N" amino acids 41 to 129 (89 residues), 72.3 bits, see alignment E=4e-24 PF09423: PhoD" amino acids 146 to 495 (350 residues), 313.6 bits, see alignment E=1.7e-97

Best Hits

KEGG orthology group: K01113, alkaline phosphatase D [EC: 3.1.3.1] (inferred from 91% identity to pfs:PFLU0807)

Predicted SEED Role

"Phosphodiesterase/alkaline phosphatase D"

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.1

Use Curated BLAST to search for 3.1.3.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166N0W3 at UniProt or InterPro

Protein Sequence (513 amino acids)

>Pf1N1B4_1143 Phosphodiesterase/alkaline phosphatase D (Pseudomonas fluorescens FW300-N1B4)
MSEFDLGRRRVMQAVGAGLLLPGLAPAVIASVKDRPQLTDGVQSGDLLGDRAMIWSRSDR
AARMVVEWDTRSLFSNPRRFVSPLADARTDFTARVELTGLPADQAIFYRVTFEDAQSGVA
SEPWFGHLRSVPTARRNIRFVWSGDTVGQGFGINPDIGGMRIYEAMRLRLPDFFIHSGDT
IYADSPVPAQLTTESGRVWRNLTSEAKSKVAETLDDYRGNYRYNLMDENIRRFNAEVPQI
WQWDDHEVVNNWSPGKQLDERYKSKDIHSLVGRARQAWLEYAPMRLQSADDGGRIYRKLS
YGPMLDVFVLDMRSYREANDDNLGAAKPFLGREQLDWLKRELKGSQAQWKVIAADMPIGL
GVPDGEVRPGVARWEAVANGDPGPAQGREVEIAELLGFLRAQQVRNFVFLTADVHYCAAH
HYHPDRAAFQDFEPFWEFVAGPLNAGSFGPNALDKTFGPEVVFEKAPPAQNTSPFAGFQF
FGEVNIDGQTGELSVVLRDLDGVEVFERTLQPV