Protein Info for Pf1N1B4_113 in Pseudomonas fluorescens FW300-N1B4

Annotation: Cytosine deaminase (EC 3.5.4.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 414 PF07969: Amidohydro_3" amino acids 42 to 378 (337 residues), 186.7 bits, see alignment E=1.2e-58 PF01979: Amidohydro_1" amino acids 48 to 377 (330 residues), 62.1 bits, see alignment E=5.8e-21

Best Hits

Swiss-Prot: 59% identical to CODA_ECOLI: Cytosine deaminase (codA) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 95% identity to pba:PSEBR_a3897)

MetaCyc: 71% identical to cytosine deaminase monomer (Klebsiella pneumoniae)
Cytosine deaminase. [EC: 3.5.4.1]; 3.5.4.1 [EC: 3.5.4.1]; 3.5.4.1 [EC: 3.5.4.1]

Predicted SEED Role

"Cytosine deaminase (EC 3.5.4.1)" (EC 3.5.4.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.4.1

Use Curated BLAST to search for 3.5.4.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166MFL5 at UniProt or InterPro

Protein Sequence (414 amino acids)

>Pf1N1B4_113 Cytosine deaminase (EC 3.5.4.1) (Pseudomonas fluorescens FW300-N1B4)
MHIINARLRNQEGLHELHLENGLIHSIARQTEAPSLGPDDLDAGGNLVVPPFVEPHIHLD
ATLTAGEPRWNMSGTLFEGIECWGERKATITHEDTKTRAKKTIQTLAAHGIQHVRTHVDV
TDPELTALKAMLEVREESRHLIDLQIVAFPQEGIESYRNGRELMEEAIRLGADVVGGIPH
FEYTRDQGVSSVKFLMDLAERTGCLVDVHCDETDDPHSRFLEVLAEEARSRDMGSRVTAS
HTTAMGSYDNAYCAKLFRLLGHSGISFVSCPTESIHLQGRFDSFPKRRGVTRVNELLEAG
MNVCFGQDSIVDPWYPLGNGNILRVLEAGLHICHMLGYRNLQSALDLVTDNSAKAMNLGD
RYGLEQGRPANLLILSADSDYEVIRSQGLPLYSVREGKVLMKRQMPVVEFSGLS