Protein Info for Pf1N1B4_1064 in Pseudomonas fluorescens FW300-N1B4

Annotation: Additional periplasmic component NikK of nickel ECF transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 239 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details PF10670: DUF4198" amino acids 20 to 212 (193 residues), 60.4 bits, see alignment E=1.4e-20

Best Hits

KEGG orthology group: K10094, nickel transport system substrate-binding protein (inferred from 88% identity to pfo:Pfl01_0873)

Predicted SEED Role

"Additional periplasmic component NikK of nickel ECF transporter" in subsystem Transport of Nickel and Cobalt

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166MZE7 at UniProt or InterPro

Protein Sequence (239 amino acids)

>Pf1N1B4_1064 Additional periplasmic component NikK of nickel ECF transporter (Pseudomonas fluorescens FW300-N1B4)
MQHLKSLALLSLLCAGQVSAHGLWTEERRGNIEVIYGHGAEDNAFKAQKISGAWAYDVAG
KMIPVNVQRLADHARLQPLKPPAVMAVALNNGMWSQTADKKWINEGRSKVPGAIESTQTF
KYSLAIYQPGAKLPKLDQMKLLILPEVDPLTVGPGKSLPVRVLLDGKPAAGVKLVGDYRS
APDTLSTETDAEGRAQVLVRNEGLNVIAAQMEIPLKDNPDVATRGLFTSLTFLGEAHHE