Protein Info for Pf1N1B4_103 in Pseudomonas fluorescens FW300-N1B4

Annotation: Histidine permease YuiF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 438 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details transmembrane" amino acids 52 to 75 (24 residues), see Phobius details amino acids 93 to 112 (20 residues), see Phobius details amino acids 118 to 136 (19 residues), see Phobius details amino acids 145 to 170 (26 residues), see Phobius details amino acids 190 to 212 (23 residues), see Phobius details amino acids 233 to 252 (20 residues), see Phobius details amino acids 258 to 277 (20 residues), see Phobius details amino acids 289 to 309 (21 residues), see Phobius details amino acids 329 to 348 (20 residues), see Phobius details amino acids 356 to 384 (29 residues), see Phobius details amino acids 420 to 437 (18 residues), see Phobius details PF13726: Na_H_antiport_2" amino acids 2 to 86 (85 residues), 102.6 bits, see alignment E=1.6e-33 PF03553: Na_H_antiporter" amino acids 149 to 432 (284 residues), 275.9 bits, see alignment E=6.3e-86 PF06808: DctM" amino acids 185 to 389 (205 residues), 34.9 bits, see alignment E=1.3e-12

Best Hits

Swiss-Prot: 58% identical to Y2115_VIBPA: Uncharacterized membrane protein VP2115 (VP2115) from Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)

KEGG orthology group: K07084, (no description) (inferred from 94% identity to pfo:Pfl01_3769)

Predicted SEED Role

"Histidine permease YuiF"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166MFF0 at UniProt or InterPro

Protein Sequence (438 amino acids)

>Pf1N1B4_103 Histidine permease YuiF (Pseudomonas fluorescens FW300-N1B4)
MNAVIAAVGIMLILSLSRVHVVIALIVGALVGGLTGGLGIDATLKAFNSGLGGGATVALS
YALLGAFAVAIAKSGLAHALADRALAMVDRQHANGGGNVKWLLIGLLWVVAIASQNILPI
HIAFIPLLVPPLLYVLTKLQLDRRLIACVITFGLITPYMFLPVGFGNIFLNEILLANVAR
SGVDISGINVTHAMGIPALGMVFGLAAAFISYRKKRVYDLEKIEQVEQVAVQYNPMSLVI
AGLAIAAAFIVQLLLDSMIIGALAGFLIFSVSGIVKWRDTDDLFTEGMKMMAMIGFIMIA
ASGFGEVIKATGQVQTLVETSASWIGHSKAIGALLMLLVGLLVTMGIGSSFSTVPILAVI
FVPLCVQLGFSPIAIVCIVGTAGALGDAGSPASDSTLGPTSGLNIDGQHHHIWDTVVPTF
LHYNLPLLVFGWVAAMVL