Protein Info for Pf1N1B4_1023 in Pseudomonas fluorescens FW300-N1B4

Annotation: Maleylacetoacetate isomerase (EC 5.2.1.2) @ Glutathione S-transferase, zeta (EC 2.5.1.18)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02798: GST_N
  • amino acids 2 to 77 (76 residues), 49.4 bits, E=9.7e-17
• TIGR01262: maleylacetoacetate isomerase
  • amino acids 3 to 210 (208 residues), 272.6 bits, E=1.1e-85
• PF13417: GST_N_3
  • amino acids 3 to 82 (80 residues), 61.4 bits, E=1.6e-20
• PF13409: GST_N_2
  • amino acids 11 to 79 (69 residues), 59 bits, E=1.1e-19
• PF13410: GST_C_2
  • amino acids 139 to 188 (50 residues), 31 bits, E=4.5e-11

Best Hits

Swiss-Prot: 80% identical to MAAI_PSEAE: Maleylacetoacetate isomerase (maiA) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K01800, maleylacetoacetate isomerase [EC: 5.2.1.2] (inferred from 89% identity to pfo:Pfl01_0912)

MetaCyc: 45% identical to glutathione transferase subunit (Escherichia coli B)
Glutathione transferase. [EC: 2.5.1.18]

Predicted SEED Role

"Maleylacetoacetate isomerase (EC 5.2.1.2) @ Glutathione S-transferase, zeta (EC 2.5.1.18)" (EC 2.5.1.18, EC 5.2.1.2)

MetaCyc Pathways

• L-tyrosine degradation I (5/5 steps found)
• 4-hydroxy-2-nonenal detoxification (1/4 steps found)
• glutathione-mediated detoxification I (3/8 steps found)
• pentachlorophenol degradation (3/10 steps found)
• gliotoxin biosynthesis (2/9 steps found)
• glutathione-mediated detoxification II (1/9 steps found)
• indole glucosinolate activation (intact plant cell) (3/12 steps found)
• camalexin biosynthesis (2/12 steps found)

KEGG Metabolic Maps

• Drug metabolism - cytochrome P450
• Glutathione metabolism
• Metabolism of xenobiotics by cytochrome P450
• Styrene degradation
• Tyrosine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.18

Use Curated BLAST to search for 2.5.1.18 or 5.2.1.2

Sequence Analysis Tools

See A0A166MYJ7 at UniProt or InterPro

Protein Sequence (211 amino acids)

>Pf1N1B4_1023 Maleylacetoacetate isomerase (EC 5.2.1.2) @ Glutathione S-transferase, zeta (EC 2.5.1.18) (Pseudomonas fluorescens FW300-N1B4)
MELYTYYRSTSSYRVRIALALKGLDYQALPINLIAPQGGEHRQPTYLGINPQGRVPALRT
DEGELLIQSPAIIEYLEERYPQVPLLSKDLVARAHERGVAAVIGCDVHPLHNVSVLNQLR
QLGHDEPQVVEWIGHWISQGLATVEQLIGDTGYCFGDQPGLADVYLIPQLYAAERFNISL
EAYPRIRRVAALAATHPAFFLAHPANQPDTP